3d Raft Culture
3d Raft Culture - Related Videos
04:43
Esophageal 3D Raft Culture Model: An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model
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2023
This video describes an in vitro culture technique to generate human organotypic esophageal constructs by co-culturing primary human esophageal fibroblast and squamous epithelial cells within a decellularized porcine scaffold. These 3D culture models mimic the function and physiology of their in vivo tissue counterparts.
07:26
Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
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Cited by 47 •
2012
An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
10:54
Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization
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2015
An efficient, three-step synthesis of RAFT-based fluorescent glycopolymers, consisting of glycomonomer preparation, copolymerization, and post-modification, is demonstrated. This protocol can be used to prepare RAFT-based statistical glycopolymers with desired structures.
10:59
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
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Cited by 9 •
2012
A technique to probe the lipid raft partitioning of fluorescent proteins at the plasma membrane of living cells is described. It takes advantage of the disparity in diffusion times of proteins located inside or outside of lipid rafts. Acquisition can be performed dynamically in control conditions or after drug addition.
04:41
Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts
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2023
This video explains the 3D magnetic bioprinting of co-cultured pancreatic cancer cells and fibroblasts. Pancreatic cancer cells and activated pancreatic fibroblast cells are co-cultured and magnetized using a biocompatible nanoparticle assembly, to generate spheroids, under the influence of magnetic forces.
FAQs
The air-liquid interface provides a condition that supports epithelial or tumor cells as they form multilayered structures rather than remaining in a single flat layer. It also promotes differentiation and tissue-like organization. In cancer studies, this organization helps investigators examine how malignant cells grow within a structured environment and how their behavior changes as the culture develops.
The collagen-based matrix provides a physical substrate for epithelial or tumor cells and helps reproduce cell-matrix interactions that are limited in conventional two-dimensional cultures. Because cancer cells grow in contact with this tissue-like support, researchers can assess architectural organization and invasion within a more realistic extracellular setting than a flat culture surface allows.
Fibroblasts can support the surrounding stromal component of the model, allowing researchers to examine interactions between malignant cells and nonmalignant supporting cells. This added context is important because tumor behavior is influenced not only by cancer cells themselves, but also by their surrounding stroma. The resulting system can help clarify mechanisms of tumor growth and invasion.
A typical setup places epithelial or tumor cells on a collagen-based matrix, often with fibroblast support, and maintains the construct at an air-liquid interface. These conditions allow the cells to develop multilayered architecture and differentiation. The resulting raft can then be examined for tissue organization, tumor growth, invasion, or treatment response within the reconstructed culture environment.
Researchers can evaluate tumor growth, epithelial organization, invasion, and interactions between malignant cells and the surrounding stroma. The multilayered structure makes it possible to study these features together rather than as isolated behaviors in a flat cell layer. Findings can provide insight into disease mechanisms and reveal how cancer cells respond within a tissue-like setting.
The model can be used to examine responses to anticancer treatments in a tissue-like environment that includes cell-matrix interactions and, when present, fibroblast-supported stromal context. Researchers may compare how malignant cells behave before and after treatment, focusing on growth, organization, or invasion. This supports evaluation of therapies and may help guide development of anticancer approaches.