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Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization
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Chemistry
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JoVE Journal Chemistry
Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization

Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization

DOI:
10.3791/52922-v

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10:54 min

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June 19, 2015

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, , , , ,
1Department of Biochemistry,University of Missouri, 2Department of Biological Sciences,University of Missouri, 3Department of Molecular Microbiology & Immunology,University of Missouri, 4Department of Child Health,University of Missouri

Chapters

  • 00:05Title
  • 02:07Synthesis of a Glycomonomer: 2-Lactobionamidoethyl Methacrylamide (LAEMA)
  • 04:26Synthesis of a Glycopolymer Using Reversible Addition-fragmentation Chain-transfer (Raft) Based Polymerization
  • 06:38Fluorescent Labeling of the Glycopolymer
  • 07:46Assay to Determine the Binding of Glycopolymer to Lectin-coated Beads
  • 09:12Results: Characteristics of Glycopolymers Produced by the RAFT Methodology
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

An efficient, three-step synthesis of RAFT-based fluorescent glycopolymers, consisting of glycomonomer preparation, copolymerization, and post-modification, is demonstrated. This protocol can be used to prepare RAFT-based statistical glycopolymers with desired structures.

Tags

Keywords: Fluorescent GlycopolymersRAFT PolymerizationGlycomonomers2-lactobionamidoethyl Methacrylamide2-gluconamidoethyl MethacrylamideN-(2-hydroxyethyl) Acrylamide(4-cyanopentanoic Acid)-4-dithiobenzoateCarboxyfluorescein Succinimidyl EsterLectin-bindingGlycoepitope

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