Open Inguinal Surgery
Open Inguinal Surgery - Related Videos
07:34
Intravital Microscopy of the Inguinal Lymph Node
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Cited by 12 •
2011
A technique for performing intravital microscopy of the inguinal lymph node (LN) is outlined. Such technique allows for real-time, in vivo study of the lymph node microvasculature and structure both during homeostasis and infection. This technique can be adapted to cell trafficking studies and to other lymph node sites.
04:22
Mouse Inguinal Lymph Node Volume Analysis: An Ultra-high-frequency Ultrasound Imaging Technique to Determine the Volume of Inguinal Lymph Node in Mouse Model
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2025
In this video, we demonstrate the technique to image the inguinal lymph node in a mouse model using ultra-high-frequency ultrasound imaging. This non-invasive imaging technique allows the monitoring of the change in lymph node volume in the animal over time.
02:29
Harvesting an Inguinal Draining Lymph Node from a Murine Model
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2025
This video demonstrates the surgical isolation procedure of the inguinal draining lymph node from a mouse model. Incision of the abdominal region provides access to the inguinal lymph node, which is surgically harvested. The harvested translucent inguinal lymph node can be used for immunological studies.
04:36
Murine Superficial Lymph Node Surgery
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Cited by 10 •
2012
To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.
07:59
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
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Cited by 10 •
2017
This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.
FAQs
Direct visualization lets the surgeon separate tissue layers while identifying relevant vessels, nerves, abnormal tissue, and inguinal lymph nodes in the operative field. This anatomical control supports targeted removal and helps document the specific tissue collected. In cancer-related procedures, it also allows the specimen’s location and appearance to be considered during pathological examination.
The groin contains multiple tissue layers and nearby structures that must be recognized as the surgeon advances toward the inguinal canal or lymph nodes. Separating layers under direct view helps organize access to the intended region, while identifying vessels and nerves clarifies the operative anatomy. These steps are central to removing abnormal tissue or nodes for evaluation.
Inguinal lymph nodes can provide evidence about whether malignancy has reached the regional drainage area. After removal, pathological examination of the nodes supports assessment of regional disease and possible metastatic spread. That information can help place the cancer in its regional context and contribute to decisions about subsequent treatment planning.
The procedure begins with a groin incision to reach the inguinal canal, lymph nodes, or nearby tissue. The surgeon then separates the tissue layers, identifies relevant vessels and nerves, and removes the selected abnormal tissue or nodes. The collected material is sent for pathological examination, creating a direct link between the operation and disease assessment.
Surgical specimens can serve purposes beyond routine pathological examination. Tissue removed from the groin may be made available for molecular and translational studies, allowing investigators to examine disease material obtained directly from an affected region. This connection between operative sampling and laboratory research helps relate regional disease findings to broader efforts in cancer biology and care.
The approach is relevant when a malignancy involves the groin or when inguinal lymph nodes may provide important regional information. Clinically, findings can support staging, evaluation of metastatic spread, and treatment planning. In research, the same operative specimens can contribute to pathological, molecular, and translational investigations focused on the involved disease region.