Retroviral Gene Transfer
Retroviral Gene Transfer - Related Videos
03:36
Production of Retroviral Particles for Gene Transfer
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2026
Source: Zjablovskaja, P., et al., Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells. J. Vis. Exp. (2018)This video demonstrates the production of recombinant retroviral particles by transfecting human kidney-derived packaging cells, enabling stable gene delivery to target cells for downstream applications such as genetic modification, functional assays, or lineage differentiation studies.
03:02
Retroviral Mediated Gene Transduction: A Gene Transfer Technique to Deliver a Transgene in Cultured Cells Using Engineered Retroviral Vectors
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2025
In this video, we demonstrate magnetically-guided transfection of plasmid DNA in primary neuronal cell culture. Magnetofection uses an external magnetic field to guide the delivery of plasmids bound to magnetic nanoparticles into cell cytoplasm.
04:59
Retroviral-Mediated Transduction: A Method to Introduce Target Gene into Cancer Cells
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2023
This video describes the protocol for genetic validation by transferring target genes using retroviruses in chronic myeloid leukemia cell lines. The retroviruses act as a mediator to transfer genetic material into the leukemia cell through the process called transduction, which enables long term expression of target gene.This method can be applied to any target cells for genetic validation and therapeutic development.
01:27
Horizontal Gene Transfer
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2025
Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...
10:14
Retroviral Transduction of T-cell Receptors in Mouse T-cells
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Cited by 21 •
2010
We present a protocol to produce antigen-specific mouse T-cells using retroviral...
FAQs
After the engineered vector enters a target cell, its RNA genome serves as the template for reverse transcription, which produces DNA. That DNA can integrate into the host genome rather than remaining separate from it. Integration establishes a genetic change that can remain active as the cell divides, making the system suitable for long-term experimental manipulation.
Integration links the transferred sequence to the cell’s host genome. As modified cells divide, the sequence can persist, allowing investigators to examine the continuing effects of an oncogene or tumor-suppressor gene rather than observing only a short-lived response. This persistence supports cancer models in which a genetic alteration is followed through cellular growth and tumor-related investigation.
Stable expression depends on a linked sequence of events: an engineered vector must enter the target cell, its RNA must be reverse-transcribed into DNA, and that DNA must integrate into the host genome. When these events occur, cell division does not necessarily eliminate the transferred sequence. Vector design and successful entry are therefore central to durable experimental gene delivery.
Researchers can place an oncogene-related sequence into an engineered vector and introduce it into target cells. Once the sequence is maintained through integration and cell division, investigators can examine how sustained oncogene activity changes the cells. This approach helps create experimental systems for connecting a defined genetic alteration with cancer-associated cellular behavior.
Researchers can modify cells with a transferred tumor-suppressor sequence and then investigate the consequences of its expression in a cancer research model. Because the sequence can persist as cells divide, the system supports examination of its effects across continuing cell populations. It therefore provides a way to study tumor-suppressor activity alongside other cancer-associated genetic changes.
The method can modify immune cells with genetic sequences intended for experimental cancer therapies. Durable delivery matters because the transferred sequence can persist while modified cells divide, supporting investigation of genetically engineered cell treatments over time. In this context, retroviral gene transfer connects stable genetic modification with research evaluating altered immune cells within cancer-focused therapeutic strategies.