Endarterectomy

Endarterectomy is a surgical procedure that removes atherosclerotic plaque from the inner lining of an artery to restore blood flow and reduce the risk of ischemic complications. During the operation, the surgeon exposes and temporarily controls the affected vessel, opens the arterial wall, and carefully excises the plaque before closing the artery, sometimes with a patch to widen the lumen. Carotid endarterectomy is the most established clinical application and can help prevent stroke in selected patients with significant carotid stenosis. The procedure also illustrates how direct removal of obstructive lesions can complement medical management of vascular disease.

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Research

JoVE Journal - Biology

Single Nuclei Isolation from Coronary Endarterectomy Tissue of Coronary Artery Bypass Graft Patients

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2026

Coronary endarterectomy tissue samples were processed to isolate single nuclei using an optimized dissociation protocol. The integrity of the isolated nuclei was validated through three complementary approaches: trypan blue exclusion staining to assess membrane integrity, DAPI-based confocal microscopy to confirm nuclear morphology, and flow cytometry to quantify nuclear yield and purity.

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

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Cited by 13 •

2014

Atherosclerosis is a chronic inflammatory process. This manuscript illustrates an easy to use ex vivo model to investigate fresh carotid or coronary artery plaques. The ex vivo model allows for the investigation of potential substances on the inflammatory milieu in human atherosclerotic lesions and results can be analyzed by various methods.

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