Spectral Acquisition

Spectral acquisition is the process of measuring and recording signal intensity across wavelengths or frequencies to characterize how a material, device, or environment interacts with electromagnetic radiation. In engineering, an instrument separates the incoming signal into spectral components, directs them to a detector, and converts the response into a calibrated spectrum whose resolution and range depend on the optics, sensor, and acquisition settings. These data support material identification, chemical and process monitoring, fault detection, remote sensing, and performance evaluation of optical and electronic systems, while careful calibration and noise control improve measurement reliability and enable comparison across experiments.

Spectral Acquisition - Related Videos

Research

JoVE Journal - Immunology and Infection

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

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2025

Spectral Iterative Bleaching Extends Multiplexity (IBEX) builds upon the base IBEX technique by adding heparin blocking to minimize nonspecific binding and leveraging spectral detection with computational unmixing to suppress autofluorescence. This approach accelerates image acquisition while reducing sources of background, enabling robust multi-round, high-parameter spatial proteomic analyses.

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

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Cited by 24 •

2013

Photoacoustic ophthalmology (PAOM), an optical-absorption-based imaging modality, provides the complementary evaluation of the retina to the currently available ophthalmic imaging technologies. We report the using of PAOM integrated with spectral-domain optical coherence tomography (SD-OCT) for simultaneous multimodal retinal imaging in rats.

Using Spectral Reflectometry to Determine Myelinated Axon Diameters in a Fixed Mouse Brain Slice

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2025

Source: Kwon, J., et al., Spectral Reflectometric Microscopy on Myelinated Axons In Situ. J. Vis. Exp. (2018) This video demonstrates the use of spectral reflectometry with a hyperspectral confocal microscope to analyze interference patterns and determine myelinated axon diameters in fixed brain tissue slices.

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

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Cited by 6 •

2011

By employing a spectrally resolved two-photon microscopy imaging system, pixel-level maps of Förster Resonance Energy Transfer (FRET) efficiencies are obtained for cells expressing membrane receptors hypothesized to form homo-oligomeric complexes. From the FRET efficiency maps, we are able to estimate stoichiometric information about the oligomer complex under study.

Research

JoVE Journal - Environment
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RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

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Cited by 36 •

2017

An experimental protocol is presented for assessment of soil grown plant root systems with RGB and hyperspectral imaging. Combination of RGB image time series with chemometric information from hyperspectral scans optimizes insights into plant root dynamics.

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