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January 19, 2011
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Summary
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By employing a spectrally resolved two-photon microscopy imaging system, pixel-level maps of Förster Resonance Energy Transfer (FRET) efficiencies are obtained for cells expressing membrane receptors hypothesized to form homo-oligomeric complexes. From the FRET efficiency maps, we are able to estimate stoichiometric information about the oligomer complex under study.
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Cite this Article
Stoneman, M., Singh, D., Raicu, V. In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy. J. Vis. Exp. (47), e2247, doi:10.3791/2247 (2011).
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