Substrate Cleaning
Substrate Cleaning - Related Videos
07:30
Cleaning Glassware
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2023
Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA Organic synthesis is about transforming a readily available reagent into a more valuable product. Having clean glassware is crucial for the efficiency of this process. Dirty glassware can potentially affect the reaction and make isolation of the final product more challenging. Thus, a synthetic chemist must keep the glassware spotless. The methods described here will detail different glass cleaning...
04:57
Proper Care and Cleaning of the Microscope
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Cited by 2 •
2008
Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.
01:30
Cleaning, Sterilization, and Disinfection
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2023
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease. Cleaning The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
11:13
Identification of Kinase-substrate Pairs Using High Throughput Screening
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Cited by 1 •
2015
Protein phosphorylation is a central feature of how cells interpret and respond to information in their extracellular milieu. Here, we present a high throughput screening protocol using kinases purified from mammalian cells to rapidly identify kinases that phosphorylate a substrate(s) of interest.
04:19
Selective Cleaning of Caenorhabditis Worms for Enrichment of Intestinal Microbiota
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2026
Source: Morgan, E., et al. Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria. J. Vis. Exp. (2021)This video demonstrates a protocol for isolating intestinal bacteria of interest from wild Caenorhabditis worms. The worms are first starved and washed to eliminate residual feeding bacteria from their surface and intestinal lumen while preserving the target microbes. After surface cleaning and multiple crawling steps, the worms are dissected to extract...
FAQs
Solvent dissolution removes contaminants that can dissolve in the selected solvent, whereas aqueous cleaning supports removal through water-based chemistry. Abrasion acts mechanically on the surface, and ultrasonic agitation adds physical energy to aid removal. Plasma treatment provides another option when the required surface condition or contamination cannot be addressed with simpler chemical or mechanical approaches.
Surface energy matters because it can influence adhesion and coating uniformity during later processing. Removing oils, particles, oxides, or residues helps prepare the surface for more consistent interaction with coatings, bonding materials, or deposited layers. In engineering applications, this improved condition can reduce defects and support more reliable performance in the finished component.
No single cleaning method suits every substrate or contaminant. Engineers compare solvent, aqueous, abrasive, ultrasonic, and plasma approaches according to material compatibility, contamination type, required surface condition, environmental constraints, and the performance demands of the finished component. Combining chemical and physical mechanisms may be appropriate when one mechanism alone cannot provide the required level of surface preparation.
A practical workflow starts by identifying the substrate, the contamination type, and the surface condition required by the next engineering step. Engineers then compare solvent, aqueous, abrasive, ultrasonic, or plasma options against compatibility and environmental constraints. This selection-focused approach helps match the treatment to the finished component’s performance demands rather than applying one universal procedure.
Substrate Cleaning is used before processes in which surface contaminants could interfere with coating, bonding, deposition, or fabrication. The selected approach depends on what the subsequent process requires from the surface, including adequate adhesion, coating uniformity, and low defect levels. This makes cleaning relevant across preparation stages for engineered components rather than limited to one manufacturing operation.
Adequacy is judged by whether the prepared surface supports the outcomes required by the next process and the finished component. Relevant indicators include improved surface energy, stronger adhesion, more uniform coatings, greater process reliability, and fewer defects associated with oils, particles, oxides, or residues. The standard therefore depends on the application’s performance demands, not cleaning alone.