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JoVE Journal
Biology

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Identification of Kinase-substrate Pairs Using High Throughput Screening
 

Identification of Kinase-substrate Pairs Using High Throughput Screening

Article DOI: 10.3791/53152
August 29th, 2015

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Summary August 29th, 2015

Protein phosphorylation is a central feature of how cells interpret and respond to information in their extracellular milieu. Here, we present a high throughput screening protocol using kinases purified from mammalian cells to rapidly identify kinases that phosphorylate a substrate(s) of interest.

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