Tcbs Agar Comparison

TCBS agar comparison evaluates how thiosulfate-citrate-bile salts-sucrose agar performs relative to other selective and differential media for recovering Vibrio species from clinical, food, or environmental samples. Its alkaline formulation, bile salts, and citrate suppress many competing microorganisms, while sucrose fermentation changes the indicator color and helps distinguish colonies by biochemical behavior; thiosulfate and ferric ions can also reveal hydrogen sulfide production. In immunology and infection research, comparing TCBS with alternative media helps assess selectivity, recovery, and interpretability during Vibrio detection. These results support pathogen surveillance, outbreak investigation, and studies of gastrointestinal and wound-associated infections.

Tcbs Agar Comparison - Related Videos

Research

JoVE Journal - Immunology and Infection

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Cited by 16 •

2016

Detection and isolation of clinically relevant Vibrio species require selective and differential culture media. This study evaluated the ability of a new chromogenic medium to detect and identify V. parahaemolyticus and other related species. The new medium was found to have better sensitivity and specificity than the conventional medium.

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We describe a qualitative assay for yeast adhesion and agar invasion as a measure of invasive and pseudohyphal differentiation. This simple assay can be used to assess the invasive phenotype of various mutants as well as the effects environmental cues and signaling pathways on yeast differentiation.

Subculturing Sulfite-Reducing Anaerobic Bacteria from an Agar Culture

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2025

Source: Anwar, Z., et al. Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples. J. Vis. Exp. (2019).In this video, sulfite-reducing bacteria are cultured in a sulfite-containing agar medium, where they grow anaerobically and reduce sulfite to hydrogen sulfide, forming black precipitates with iron ions. Black colonies are isolated from the agar depth using aseptic techniques and transferred to fresh medium for continued anaerobic incubation.

Education

JoVE Core - Social Psychology

The Sense of Self: Reflected Self-Appraisal and Social Comparison

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2020

According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...

Agar-Block Microcosms for Controlled Plant Tissue Decomposition by Aerobic Fungi

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Cited by 6 •

2011

This video demonstrates a controlled environment approach to study degradation of lignocellulosic plant tissues by aerobic fungi. The ability to control nutrient sources and moisture is a key advantage of agar-block microcosms, but the approach often yields mixed success. We address critical pitfalls to yield reproducible, low-variability results.

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