Canine Neutrophil Isolation
Canine Neutrophil Isolation - Related Videos
06:45
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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Cited by 8 •
2016
Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.
07:12
Neutrophil Isolation Protocol
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Cited by 173 •
2008
Neutrophils are among the first cells to arrive on the site of inflammatory immune response, and their functions and mechanisms have been studied extensively in vitro. We demonstrate a standard density gradient separation method to isolate human neutrophils from whole blood using commercially available separation media.
09:37
Isolation and Expansion of Adult Canine Hippocampal Neural Precursors
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Cited by 3 •
2016
The canine brain is a valuable model in which to study adult neurogenesis. Presented here are protocols for isolating and expanding adult canine hippocampal neural precursor cells from primary brain tissue.
08:24
Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
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Cited by 11 •
2016
Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.
04:50
Isolation and Digestion of Neutrophil Proteins
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2026
Source:Tia Rizakos1, Jennifer Geddes-McAlister11Molecular and Cellular Biology Department, University of Guelph.This video demonstrates the protein isolation from neutrophils, which is further processed and digested into smaller peptides. These peptides can be used for future analysis.
FAQs
Layered centrifugation separates blood components because they differ in cell density. In canine neutrophil isolation, this physical difference allows the neutrophil fraction to be recovered apart from erythrocytes, mononuclear leukocytes, and plasma. The separation principle is important because it provides a defined cell population for controlled immunology and infection assays.
Removing erythrocytes, mononuclear leukocytes, and plasma reduces cellular and fluid components that could interfere with neutrophil-focused measurements. Recovering the appropriate fraction and washing it helps produce a preparation enriched for neutrophils rather than a mixed blood sample. This improves the relevance of subsequent measurements of phagocytosis, antimicrobial activity, oxidative burst, and cytokine interactions.
Careful handling helps preserve neutrophil viability and functional responsiveness after separation. This matters because the cells must remain sufficiently intact and responsive to produce meaningful results in controlled assays. Poor preservation could weaken or alter measured immune activities, making it harder to distinguish genuine differences in antimicrobial responses from changes caused by the isolation process itself.
The main workflow begins with dog blood and uses layered centrifugation to separate components according to density. The neutrophil-containing fraction is then recovered and washed to remove remaining plasma and unwanted cells. The resulting preparation can be assessed or used in functional experiments, with handling focused on maintaining viability and responsiveness throughout the procedure.
Isolated canine neutrophils support controlled studies of several innate immune functions, including phagocytosis, antimicrobial responses, oxidative burst, and cytokine interactions. Because the cells are examined outside the original mixed blood environment, researchers can focus on neutrophil behavior and compare responses under defined experimental conditions. These readouts help characterize cellular activity during veterinary immunology studies.
The method is useful when researchers need to investigate canine host-pathogen mechanisms, disease-related immune changes, or responses to immune-modulating treatments. Isolated cells provide a focused system for examining how neutrophils contribute to innate defense. Results can help evaluate disease processes and treatment effects while limiting interpretation to functional responses measured in the recovered cell population.