Diaphragm Paralysis

Diaphragm paralysis is the partial or complete loss of movement in one or both diaphragms, the primary muscles that drive breathing. It usually results from impaired phrenic nerve signaling, muscle disease, or injury, preventing normal diaphragm contraction and reducing ventilation; unilateral paralysis may cause few symptoms, whereas bilateral paralysis can produce substantial breathing difficulty, particularly when lying down. Diagnosis commonly combines physical examination with chest imaging, pulmonary function testing, and assessments of diaphragm motion. Management depends on the cause and severity and may include observation, treatment of underlying disease, respiratory support, or surgical interventions, making recognition important in clinical medicine.

Diaphragm Paralysis - Related Videos

Research

JoVE EoE - Assay Techniques

Fluorescent Reporter-Based Paralysis Assay: A Technique to Assess Age-Associated Progressive Formation of Polyglutamine Fluorescent Reporter and Associated Paralysis in Caenorhabditis elegans

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2025

This video demonstrates an in vivo imaging-based assay to determine proteostasis decline due to aging. The assay uses Caenorhabditis elegans expressing polyglutamine YFP-fusion protein in body wall muscles to measure the age-associated aggregation of polyglutamine and induction of proteotoxicity, leading to paralysis.

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function

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Cited by 7 •

2014

Diagnostic ultrasound imaging has proven to be effective in diagnosing various respiratory diseases in human and animal subjects. We demonstrate a comprehensive ultrasound protocol utilized by Dr. Zuo's lab to analyze diaphragm kinetics specifically in mouse models. This is also a non-invasive research technique which can provide quantitative information on mouse respiratory muscle function.

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

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Cited by 16 •

2015

Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.

Fluorescence Imaging of Calcium Dynamics at Neuromuscular Junctions in the Diaphragm of a Transgenic Mouse

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2025

Source: Heredia, D. J., et. al. Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm. J. Vis. Exp. (2018) This video demonstrates fluorescence imaging of calcium dynamics at neuromuscular junctions (NMJs) in a transgenic mouse expressing genetically encoded calcium indicators. A NJM is identified by fluorescently tagged acetylcholine receptors in the diaphragm with the phrenic nerve. An image splitter is used to visualize multiple signals...

Inducing Paralysis in a Mouse Model via Transfer of Aquaporin-4-Specific Th17 Cells

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2025

This video demonstrates the procedure of inducing paralysis by transferring activated Th17 cells targeting aquaporin-4 into a mouse model. In the central nervous system, the activated Th17 cells interact with aquaporin-4-expressing astrocytes, triggering an immune response that leads to tail and limb paralysis in the mouse.

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