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Neuroscience

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JoVE Journal Neuroscience
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
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Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Article DOI: 10.3791/52605
May 25th, 2015 •
1,2, 1, 2, 1
1Department of Neuroscience, Farber Institute for Neurosciences, Sidney Kimmel Medical College at Thomas Jefferson University, 2Department of Biology, Arcadia University

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May 25th, 2015

Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.

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