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Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
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JoVE Journal Neuroscience
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

DOI:
10.3791/52605-v

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09:43 min

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May 25, 2015

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, , ,
1Department of Neuroscience, Farber Institute for Neurosciences,Sidney Kimmel Medical College at Thomas Jefferson University, 2Department of Biology,Arcadia University

Chapters

  • 00:05Title
  • 01:40Preparations
  • 02:43Compound Muscle Action Potential (CMAP) Recording
  • 03:28Isolating and Staining the Diaphram
  • 06:06Imaging and NMJ Analysis
  • 08:00Abnormal Peak CMAP Measurements and NMJ Morphologies
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.

Tags

Keywords: Diaphragm InnervationPhrenic Motor NeuronsCompound Muscle Action PotentialNeuromuscular JunctionImmunohistochemistryDenervationReinnervationRodent ModelsCNS DiseasePNS Disease

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