Heterotopic Cervical Model
Heterotopic Cervical Model - Related Videos
09:48
Heterotopic Cervical Heart Transplantation in Mice
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Cited by 24 •
2015
The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.
10:32
Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
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Cited by 26 •
2014
The murine cervical heart transplantation model is well suited for immunological as well as ischemia reperfusion injury studies. We modified the procedure using a non-suture cuff technique and performed more than 1,000 successful transplants with this approach. Herein, we provide additional details of this technique to supplement the video.
09:10
Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease
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Cited by 7 •
2010
This video shows and compares two experimental models to study the development of obliterative airway disease (OAD) in mice, the heterotopic and orthotopic tracheal transplantation model.
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
06:45
A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
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Cited by 1 •
2022
In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.
FAQs
The graft depends on surgical connections between its vessels and the recipient’s cervical blood vessels. These vascular anastomoses establish circulation through the transplanted organ, allowing investigators to evaluate whether the graft remains active and survives. Because the model preserves the recipient’s native organ, graft performance can be studied without making the transplant responsible for the recipient’s essential organ function.
A heterotopic cervical model places the donor organ in a separate anatomical location while leaving the recipient’s original organ in place. This arrangement separates graft assessment from replacement of native-organ function. Consequently, investigators can examine transplantation biology, including graft activity and survival, without relying on the transplanted organ to sustain the recipient’s baseline organ function.
Once circulation reaches the graft, the model provides a setting for investigating transplant rejection, ischemia-reperfusion injury, immunosuppressive therapies, and graft function. These processes represent different aspects of transplantation biology, from injury associated with restoring blood flow to immune responses against the graft. Studying them in one model supports controlled evaluation of treatment strategies and transplant outcomes.
The essential workflow consists of transplanting the donor organ into the recipient’s neck and connecting the graft to cervical blood vessels through vascular anastomoses. The recipient’s native organ remains in place throughout the experiment. After circulation is established, investigators assess graft activity and survival, creating a controlled platform for studying transplantation-related responses.
The cervical location makes the graft accessible for investigation while keeping it separate from the recipient’s native organ. This combination helps researchers examine graft activity and survival under controlled conditions without replacing essential native-organ function. The model’s accessibility and functional separation are especially relevant when comparing transplant responses or evaluating immunosuppressive treatment strategies.
Researchers may choose this approach when they need to study transplantation biology without making the graft responsible for maintaining the recipient’s original organ function. Its applications include investigations of rejection, ischemia-reperfusion injury, immunosuppressive therapies, and graft function. The model therefore supports controlled testing of treatment strategies and assessment of how transplanted organs respond over the course of an experiment.