Lamb Model
Lamb Model - Related Videos
06:10
Following Cell-fate in E. coli After Infection by Phage Lambda
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Cited by 20 •
2011
This article describes the procedure for preparing a fluorescently-labeled version of bacteriophage lambda, infection of E. coli bacteria, following the infection outcome under the microscope, and analysis of infection results.
07:08
The Lambda Select cII Mutation Detection System
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Cited by 3 •
2018
We describe a detailed protocol for the Lambda Select cII mutation assay in cultured cells of transgenic rodents or the corresponding animals treated with a chemical/physical agent of interest. This approach has been widely used for mutagenicity testing of carcinogens in mammalian cells.
02:21
Generation of a Murine Model with a Central Nervous System Infection
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2025
This video demonstrates the generation of a mouse model with a CNS infection. A suckling pup is injected with the virus both intracranially and intraperitoneally. The virus spreads, infecting the CNS causing neuronal cell death and inflammation. The pup is assessed for symptoms of infection as the disease progresses.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
03:05
Establishing a Focal Demyelination Mouse Model via a Two-Point Injection of Lysophosphatidylcholine
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2025
This video demonstrates the generation of a focal demyelination mouse model using a two-point Lysophosphatidylcholine (LPC) injection. The skull of an anesthetized mouse is exposed. The injection coordinates are determined, and a hole is drilled to enable LPC injections at targeted sites. LPC induces focal demyelination of neurons via inflammation, while a two-point injection ensures its sustainability.
FAQs
Developmental timing allows researchers to examine how organ function changes before and after birth. Lambs provide measurable fetal and neonatal changes, so investigators can introduce a defined intervention at a selected stage and monitor physiological responses over time. This supports studies of development, premature birth, and neonatal care rather than limiting observations to a single experimental time point.
The larger body size provides greater access for surgical procedures, device evaluation, and repeated sampling. These capabilities can be difficult to achieve in smaller laboratory animals, particularly when investigators need to perform defined fetal interventions or follow organ function longitudinally. As a result, the model can support experimental designs that require clinically relevant access and ongoing measurements.
Investigators can monitor organ function and compare physiological outcomes with clinically relevant measurements. The model is particularly useful when fetal or neonatal changes need to be tracked over time, including responses associated with respiratory and cardiovascular function. These observations help characterize how an intervention affects developing systems under controlled experimental conditions.
Researchers can examine conditions before birth and then continue observation during neonatal life, using measurable changes across both stages. This makes it possible to relate fetal interventions to later physiological outcomes and to study problems associated with premature birth or neonatal care. The approach connects developmental biology with medical evaluation over a clinically important period.
A study generally begins by selecting a fetal or neonatal condition and applying a defined intervention under controlled experimental conditions. Researchers then monitor organ function, collect repeated measurements when needed, and compare the resulting physiological outcomes with clinically relevant measures. The exact intervention and monitoring plan depend on whether the investigation addresses surgery, disease, development, or treatment.
Applications include fetal surgery, premature birth, respiratory disorders, cardiovascular function, and neonatal care. Researchers can use the model to investigate disease processes, developmental changes, and responses to medical interventions within these areas. Its combination of surgical access, measurable fetal and neonatal changes, and longitudinal monitoring makes it relevant to several forms of maternal-fetal and neonatal research.
The model allows researchers to evaluate devices, procedures, and therapeutics under controlled conditions before human studies. Investigators can perform defined interventions, monitor organ function, and assess physiological outcomes over time. This provides experimental information about how a proposed medical approach performs in a large-animal setting and helps connect preclinical findings with clinically relevant measurements.