Organ Function Preservation
Organ Function Preservation - Related Videos
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Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
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2025
Donor heart preservation is a major determinant of success in heart transplantation. Technological advances have led to optimizing the conditions of preservation, along with a more accurate assessment of the donor heart before transplantation. We herein describe two innovative technologies currently applied for cardiac preservation: uniform cooling preservation and normothermic ex-situ heart perfusion.
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Vibratome Sectioning for Enhanced Preservation of the Cytoarchitecture of the Mammalian Organ of Corti
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Cited by 13 •
2011
A simple procedure of vibratome sectioning the organ of Corti, followed by immunohistochemistry and confocal microscopy is described. This procedure allows for improved preservation of the fine cytoarchitecture of the mammalian organ of Corti, and consequently allows for accurate quantification of cell types.
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Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
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Cited by 29 •
2015
We describe a method of ex vivo machine perfusion of human liver grafts at subnormothermic temperature (21 °C).
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Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
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Cited by 17 •
2014
One key to successful investigation of microglial biology is the preservation of microglial immunofunction ex vivo during isolation from CNS tissue. Isolating microglia via rotary shaking results in highly pure and immunofunctional cell cultures as assessed by fluorescent imaging, immunocytochemistry, and ELISA following microglia activation with the proinflammatory stimuli lipopolysaccharide (LPS) and Pam3CSK4 (Pam).
15:40
An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization
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Cited by 35 •
2013
The mammalian nose is a multi-functional organ with intricate internal structures. The nasal cavity is lined with various epithelia such as olfactory, ...
FAQs
Controlled temperature and metabolic management work together to limit ischemic injury: temperature helps control physiological activity, while oxygen and nutrient delivery support the organ when normal blood flow is disrupted. Perfusion adds a means of delivering those supports, whether the organ remains in the body or is stored outside it. This coordinated control helps preserve structure, viability, and performance.
Perfusion provides a way to deliver oxygen and nutrients during periods when normal blood flow cannot adequately support an organ. In organ function preservation, it can be used as part of a broader strategy that also controls temperature and metabolic activity. This is important for maintaining viability and physiological performance in settings involving transplantation or external storage.
Ischemic injury is a central target because disrupted blood flow threatens more than one aspect of an organ’s condition. Preservation therefore aims to protect its structure, maintain viability, and sustain physiological performance at the same time. Achieving these goals can improve graft quality and help clinicians protect organs during transplantation and other periods of circulatory failure.
Key controls include temperature, oxygen and nutrient availability, perfusion, and the level of metabolic activity. Their management addresses the preservation setting, whether support occurs within the body or while an organ is stored outside it. Together, these controls address the loss of normal environmental support and help maintain function for later clinical use.
During transplantation, preservation extends the time an organ can remain supported before use and can improve graft quality. Its value is not limited to storage duration: advances may also expand donor eligibility, increasing the range of organs considered for transplantation. The intended clinical relevance is improved patient outcomes.
Beyond transplantation, clinicians may use organ function preservation to protect organs during surgery, trauma, critical illness, and recovery from circulatory failure. These settings share a risk that normal blood flow or environmental support is disrupted. Preservation strategies provide a way to manage that risk while supporting recovery of organ function.