Pre-sensitized Mice
Pre-sensitized Mice - Related Videos
10:28
Assessment of Cocaine-induced Behavioral Sensitization and Conditioned Place Preference in Mice
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Cited by 19 •
2016
This protocol is intended to enable researchers to conduct experiments designed to test these aspects of addiction using the conditioned place preference and locomotor behavioral sensitization assays.
06:23
The 4 Mountains Test: A Short Test of Spatial Memory with High Sensitivity for the Diagnosis of Pre-dementia Alzheimer's Disease
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Cited by 68 •
2016
This article describes the 4 Mountains Test (4MT), a hippocampus-dependent test of working allocentric spatial memory. The hippocampus is affected early in Alzheimer's disease (AD) and this article outlines the 4MT methodology and results of patient testing, which demonstrates the value of the 4MT in the diagnosis of pre-dementia AD.
07:49
Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus
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Cited by 105 •
2013
Electronic von Frey measurements are an effective and improved alternative to classical von Frey filaments, as they allow rapid and precise measurement of changes in rodent mechanical withdrawal thresholds to continuously applied pressure stimuli before and after an inflammatory event.
08:49
Assessing Changes in Volatile General Anesthetic Sensitivity of Mice after Local or Systemic Pharmacological Intervention
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Cited by 23 •
2013
Loss of the righting reflex has long served as a standard behavioral surrogate for unconsciousness, also called hypnosis, in laboratory animals. Alterations in volatile anesthetic sensitivity caused by pharmacological interventions can be detected with a carefully controlled high-throughput assessment system, which may be adapted for delivery of any inhaled therapeutic.
08:35
A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
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Cited by 30 •
2015
The Cold Plantar Assay (CPA) measures cold responsiveness between 30 °C and 5 °C, and can also measure cold adaptation. This protocol describes how to use the CPA to measure cold hypersensitivity, analgesia, and adaptation in mice.
FAQs
Initial exposure activates antigen-specific adaptive immunity. On later challenge, that prior immune preparation can be examined through antibody production, inflammatory signaling, or tissue changes. The value is the controlled relationship between exposure and response, allowing investigators to assess how a defined immune history shapes measurable outcomes rather than treating the later challenge as an isolated event.
A defined antigen or allergen provides a specified immune target for the initial exposure and subsequent challenge. This helps connect the experimental stimulus to antigen-specific immune activity and observed outcomes. Because the target is controlled, investigators can study relationships among exposure, antibody production, inflammatory signaling, and tissue changes with greater experimental consistency.
Prior sensitization establishes a controlled immune history before testing begins. Animals therefore enter the challenge phase with a deliberately induced state of heightened responsiveness, which can make antibody, inflammatory, or tissue outcomes more comparable across subjects. This reproducibility supports clearer evaluation of disease mechanisms, treatment effects, and safety-related findings.
The general workflow includes an initial exposure to a defined antigen or allergen, a later experimental challenge, and measurement of the resulting immune or tissue responses. Investigators may assess antibody production, inflammatory signaling, or tissue changes after re-challenge. Comparing these outcomes across experimental conditions helps link prior exposure with subsequent biological effects.
These models are useful when investigators need to examine allergy, hypersensitivity, or immune-mediated disease under reproducible conditions. They allow a later challenge to generate measurable responses associated with prior antigen exposure. As a result, researchers can investigate disease mechanisms and evaluate potential treatments in an experimental system designed to produce controlled immune responsiveness.
Treatment studies can examine whether an intervention changes antibody production, inflammatory signaling, or tissue changes after re-challenge. Those outcomes may help researchers assess therapeutic efficacy in relation to a defined immune response. The same controlled setting can also support safety evaluation by revealing how an intervention affects measurable immune or tissue consequences.