Multi Shank Linear Silicon Probe
Multi Shank Linear Silicon Probe - Related Videos
03:22
Recording Brain Electrical Activity in Behaving Mice Using Multi-Shank Linear Silicon Probes
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2025
This video outlines a protocol for implanting a multi-shank linear silicon probe into the mouse brain to measure local field potentials. The procedure involves securing the probe to a stereotaxic unit, positioning it accurately over the target brain region, inserting it into the brain, and securing it with dental cement. After implantation, the assembly is protected with a casing. Following recovery, the setup allows for recording local field potentials, providing insights into neuronal...
17:37
Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents
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Cited by 111 •
2012
We describe methods for large-scale recording of multiple single units and local field potential in behaving rodents with silicon probes. Drive fabrication, probe attachment to the drive and probe implantation processes are illustrated in sufficient details for easy replication.
05:45
Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat
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Cited by 24 •
2011
Extracellular recordings of neuronal activity using silicon probes in the anesthetized rat will be described. This technique allows information to be obtained across multiple brain areas from more than 100 neurons simultaneously. It provides information with single cell resolution about neuronal ensembles dynamics in multiple local circuits.
06:40
Insertion of Flexible Neural Probes Using Rigid Stiffeners Attached with Biodissolvable Adhesive
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Cited by 99 •
2013
Insertion of flexible neural microelectrode probes is enabled by attaching probes to rigid stiffeners with polyethylene glycol (PEG). A unique assembly process ensures uniform and repeatable attachment. After insertion into tissue, the PEG dissolves and the stiffener is extracted. An in vitro test method evaluates the technique in agarose gel.
10:35
Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy
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Cited by 26 •
2017
Here, we present a procedure for performing large-scale Ca2+ imaging with cellular-resolution across multiple cortical layers in freely moving mice. Hundreds of active cells can be observed simultaneously using a miniature, head-mounted microscope coupled with an implanted prism probe.
FAQs
Its electrode sites sense extracellular voltage fluctuations in the surrounding tissue. Nearby neuronal action potentials contribute rapid changes to this voltage, while synaptic activity contributes additional fluctuations associated with communication among neurons. Because each site samples local electrical activity, simultaneous recordings can reveal how neural signals vary across neighboring and distributed populations.
Linear site placement creates an organized sampling path across different tissue depths. This arrangement helps researchers compare activity from multiple positions within a brain structure, making depth-dependent patterns easier to examine. In particular, it supports studies of laminar processing, in which neural activity is related to the organization and function of different layers.
Several shanks allow recordings from multiple brain locations during the same experiment, while the sites on each shank sample different depths. This combines broad anatomical coverage with organized local sampling and reduces the need for separate recording electrodes. The resulting measurements can connect activity across regions and within layered tissue more directly.
Measurements from several depths help distinguish activity patterns that may be linked to particular positions within a brain structure. Comparing these signals can expose changes in processing across layers rather than treating the entire region as uniform. This is especially useful when investigating circuit organization or determining how neural activity is distributed through tissue.
The probe is inserted into brain tissue so that its shanks and electrode sites occupy selected locations and depths. The sites then record extracellular voltage fluctuations simultaneously. Researchers can organize these recordings by shank, position, and depth, allowing activity from distributed neural populations to be examined within a common neurophysiological measurement.
The recordings can show how neural activity is distributed across multiple brain locations and depths. Researchers can use these patterns to examine circuit organization, laminar processing, and interactions between brain regions. Because measurements occur simultaneously, the data support comparisons among populations that are active at different anatomical positions during the same study.
This approach is useful when a study must relate neural activity to sensory processing, behavior, or disease-related changes in network function. Its multi-location and multi-depth sampling can capture activity from distributed populations rather than a single recording position. That coverage helps researchers examine how circuit-level signals accompany specific conditions or experimental experiences.
Multiple shanks provide simultaneous access to activity from separate brain locations, creating a basis for comparing their neurophysiological signals. Researchers can examine whether patterns across regions change together during sensory processing, behavior, or disease-related network disruption. The method therefore links local extracellular measurements to broader questions about communication and organization within neural circuits.