Transmitted Light
Transmitted Light - Related Videos
10:22
Transmitting Plant Viruses Using Whiteflies
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Cited by 41 •
2013
Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...
01:26
Sexually Transmitted Infections
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2024
Sexually transmitted infections (STIs) are diseases transmitted primarily through unsafe sexual interactions. Bacteria, viruses, or parasites cause them and can result in severe health complications if untreated.ChlamydiaThe bacterium Chlamydia trachomatis is responsible for the disease Chlamydia, the most common STI in the United States. This peculiar pathogen requires human cells to reproduce, residing intracellularly. The initial infection often goes unnoticed because it typically does not...
09:00
A Multi-detection Assay for Malaria Transmitting Mosquitoes
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Cited by 17 •
2015
Malaria transmitting mosquitoes have a number of epidemiologically important characteristics that can only be detected using molecular techniques. Utilizing a MALDI-TOF based SNP genotyping platform, we developed an assay for simultaneously detecting multiple key traits (species, insecticide resistance, parasite infection and host choice) of malaria vectors.
10:57
Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children
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Cited by 58 •
2012
Chronic infection with soil-transmitted helminths (STHs) causes malabsorption, stunting, and wasting in the growing child. Hence, it is plausible that these infections also reduce the physical fitness of children. Here, we visualize two techniques for the diagnosis of STHs and the 20-meter shuttle run test for assessing children's physical fitness.
08:08
Major Components of the Light Microscope
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2008
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications, and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The components of the microscope are described in detail here.
FAQs
Image intensity changes when light is absorbed or scattered within a specimen, while greater thickness can further reduce the transmitted signal. Areas that remove more light therefore appear darker than neighboring regions. This contrast helps researchers distinguish structural features in thin or transparent preparations, even when those features are not being identified through fluorescence-based observations.
The optical path determines how the emerging signal is collected after illumination crosses the specimen. A source sends light through the preparation, and lenses gather the transmitted light for image formation. Because the collected signal reflects what remains after passage, observing this path helps reveal differences in cellular or tissue structure.
Bright-field images obtained with transmitted light can provide a structural reference when researchers compare them with fluorescence-based observations. The transmitted-light view supports assessment of overall morphology and tissue organization in preparations such as cultured neurons or tissue sections. This comparison can help place experimental observations within the broader physical structure of the sample.
Researchers direct illumination from a source through the preparation, collect the light emerging on the opposite side with lenses, and examine the resulting contrast. This workflow applies to cultured neurons, tissue sections, and other preparations. The resulting image can then support assessment of cellular morphology, tissue organization, or changes produced during an experiment.
Transmitted-light imaging is useful for examining cultured neurons, tissue sections, and other preparations in which cellular or tissue structure is important. It can reveal neuronal morphology and broader organization without relying on the contrast of a fluorescence-based image. Researchers may therefore use it to document specimen structure and evaluate changes across experimental conditions.
The images can support evaluation of cellular morphology, tissue organization, and experimental changes. In a neuronal culture, researchers may focus on visible cellular structure, whereas tissue sections can be considered in terms of larger-scale organization. Because contrast reflects differences in light transmission through the preparation, the method provides structural observations for comparison across samples or conditions.