Eosinophils
Eosinophils - Related Videos
03:23
Three-Dimensional Cell Culture Models to Investigate the Epithelial Barrier in Eosinophilic Esophagitis
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Cited by 2 •
2024
Here, a protocol for the culture of human esophageal organoids and air-liquid interface culture is provided. Esophageal organoids' air-liquid interface culture can be used to study the impact of cytokines on the esophageal epithelial barrier.
08:44
Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
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2020
Environmental allergens such as house dust mites (HDM) often contain microbial substances that activate innate immune responses to regulate allergic inflammation. The protocol presented here demonstrates the identification of dsRNA species in HDM allergens and characterization of their immunogenic activities in modulating eosinophilic lung inflammation.
11:19
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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Cited by 2 •
2016
Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.
05:54
An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
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2025
This video demonstrates an in vitro technique to identify immune cells residing in murine lungs. After isolating target immune cells from murine lung tissue, they are surface-labeled using a cocktail of fluorophore-tagged antibodies against specific markers on innate and adaptive immune cells. Additionally, monocytes, macrophages, and dendritic cells are labeled using an intracellular marker. The various immune cell types are then identified using flow cytometry.
10:28
High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence
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2025
Spectral Iterative Bleaching Extends Multiplexity (IBEX) builds upon the base IBEX technique by adding heparin blocking to minimize nonspecific binding and leveraging spectral detection with computational unmixing to suppress autofluorescence. This approach accelerates image acquisition while reducing sources of background, enabling robust multi-round, high-parameter spatial proteomic analyses.
FAQs
Interleukin-5 and eotaxins act as distinct recruitment-related signals: interleukin-5 supports eosinophil involvement, while eotaxins help direct cells toward tissues. Once recruited, their accumulation links signaling with local inflammatory effects. This distinction helps pharmacologists examine whether a response reflects altered trafficking, broader eosinophil biology, or both.
Eosinophil granule proteins, lipid mediators, and cytokines contribute to defense against parasites, but the same released substances can damage surrounding tissues and promote inflammation. Their effects therefore depend on the biological setting. Pharmacological studies examine this balance to understand how eosinophil activity contributes to airway inflammation and other tissue responses.
Interleukin-5 signaling is important because it helps regulate eosinophil participation in inflammatory responses. Inhibiting this pathway can reduce eosinophil-related activity and clarify how the cells contribute to disease. This approach supports mechanistic research in asthma, atopic disease, and other eosinophilic disorders while informing development of targeted treatments.
Eosinophil survival provides another point of pharmacological control beyond recruitment. Treatments that reduce survival can lower the persistence of these cells and help investigators test whether ongoing eosinophil activity sustains inflammation. Studying survival alongside interleukin-5 signaling separates different contributors to disease biology and may help explain variation in treatment responses.
Researchers assess eosinophils using blood counts and tissue measurements. Blood data provide one way to monitor eosinophil-related changes, whereas tissue measurements address local accumulation at affected sites. Comparing these observations can help evaluate disease activity and determine whether a pharmacological intervention changes the eosinophil response associated with inflammation.
Asthma and atopic disease are important settings because eosinophil accumulation and mediator release can contribute to airway or tissue inflammation. Pharmacological research uses these conditions to investigate disease mechanisms and evaluate interventions that inhibit interleukin-5 signaling or reduce eosinophil survival. Findings can connect cellular activity with treatment response.
Eosinophil measurements provide pharmacological research with indicators of disease activity and response to treatment. Blood counts and tissue assessments can be used alongside interventions directed at interleukin-5 signaling or eosinophil survival. This combination helps determine whether altering eosinophil biology produces measurable changes and supports development of more targeted approaches for eosinophilic disorders.