Intramusculaire (IM) injectie: samengestelde toediening in de dorsale spier van een volwassen zebravis

- Transfer a tricaine anaesthetized fish onto a wet sponge with the dorsal side facing up. Place the sponge carrying the fish under a microscope. Position a micro-injection needle at a 45 degree angle from the body axis near the dorsal muscle.

Next, locate the spot in front of the dorsal fin that is not covered with scales where you can insert the needle without applying great force. Then gently insert the needle and slowly inject the compound of interest along with a marker such as phenol red into the muscle.

If the compound of interest contains genetic material, electroporate the cells by placing electrodes on either side of the injection site and administering the current. The current makes the muscle cells permeable and able to efficiently take up the genetic material. After the procedure, immediately transfer the fish into a recovery tank. In the following protocol, we will perform an intramuscular injection on an adult zebrafish to deliver a DNA vaccine against mycobacteriosis.

To keep the fish in a fixed position during injection, use a piece of sponge with a groove cut in the middle as padding. Soak the sponge thoroughly in system water and set it in a Petri dish. Using a p