Zebrafish Brain Disissection: Een techniek van vis neurobiologie

- Place a euthanized fish on a dissection bed and use a surgical blade to decapitate the fish at the level of the gills. Hold the head with the ventral side facing up and remove the soft tissues until you see the optic chiasm, a structure made of optic nerves that connects the brain to the eyes.

Cut the optic nerves and remove the eyes. Now, orient the fish with the dorsal side facing up and remove the parts of the skull to isolate the brain. Transfer the brain into a Petri dish containing a dissection medium to maintain a constant pH of the tissue. Observe the parts of the brain.

The olfactory bulbs are a pair of structures at the anterior end connected to the olfactory organs which detect odor signals. The telencephalon includes memory related areas. The habenula relays information from the telencephalon to other parts of the brain.

The optic tectum is a sensory information processing center. The cerebellum plays a role in somatic motor function and balance. And the medulla relays information from the spinal cord to the brain. In the following protocol, we will isolate the brain from an adult zebrafish to collect neural stem cells from different regions.

To begin, prepare a disse