Staartaderinjectie: een methode om kankercellen toe te dienen voor gemetastaseerde studies in een muismodel

Start by adding trypsin into a Petri dish containing labeled breast cancer cells to detach them from the surface. Add medium containing serum to stop the action of trypsin. Transfer the cell suspension to a conical tube and centrifuge it to pellet cell.

Discard the supernatant and resuspend the cells in phosphate-buffered saline. Place the tube on ice to slow down cell metabolism. Load this cell suspension containing the required amount of cells in a Luer lock syringe and fix a needle onto it.

Place a mouse in a rodent restrainer with its tail outside. Dip the tail in warm water to dilate the veins. There are two veins on the lateral sides and an artery on the ventral side of the tail. Wipe the tail with alcohol, insert the needle, and inject the cell suspension.

Once in the bloodstream, the injected cancer cells invade into organs, such as the lungs, and proliferate. Slowly remove the needle and use a sterile gauze on the injection site to apply pressure to stop bleeding. Return the mouse to its cage and ensure a full recovery. In the following protocol, we demonstrate the injection of labeled metastatic cancer cells into a mouse model to quantify breast cancer metastasis and colo