Implantation of a Flexible Biocompatible Probe in a Glioblastoma Mouse Model

Secure a mouse in a stereotactic frame with a surgically exposed brain containing a pre-implanted glioblastoma spheroid.

Apply saline to the exposed brain to maintain tissue hydration.

Position the flexible probe over the saline layer, ensuring its contact pads rest on the mouse's back and the electrodes align above the glioblastoma.

The probe consists of a thin, flexible polymer substrate with patterned gold electrodes coated with biocompatible conductive polymer, ensuring optimal electrical performance and tissue compatibility.

Absorb excess saline, leaving only a thin layer.

Apply silicone adhesive to stabilize the electrodes, then place a cover glass.

Apply dental cement around the cover glass and secure the contact pads to the mouse's back.

Construct a cement well for fluid insertion.

Later, administer post-surgical analgesia and maintain thermal support for the mouse's recovery.

The implanted electrodes deliver electrical pulses above the glioblastoma for targeted pulsed electric field therapy in neurotherapeutic applications.

Place a drop of DPBS to cover the craniotomy. Place the flexible electrode onto the drop of DPBS and gently place the back of the probe with the contact pads ont