Stereotaxic Transplantation of Neural Cell Suspension into a Mouse with Traumatic Brain Injury

Begin with a preloaded syringe attached to a stereotaxic syringe pump.

The syringe contains a human-induced pluripotent stem cell-derived neural cell suspension.

Position the needle above the surgically exposed brain cortex of a mouse with traumatic brain injury.

Lower the syringe to touch the dura with the needle tip, adjust the coordinates to target the injury site, and retract slightly. Now,

create a small incision, establishing an entry point for the needle.

Next, lower the plunger to allow the flow of the cell suspension.

Lower the needle and penetrate the brain to reach the gray matter-white matter border of the deep cortex.

Finally, slowly infuse the cell suspension while irrigating the surgical site with saline to maintain hydration.

Upon completion, withdraw the needle, re-irrigate, and close the incision.

Over time, the transplanted cells mature, restore neural circuits, and repair brain damage.

Roughly 24 hours after the craniectomy, place the mouse back into the stereotaxic frame.

Cover the mouse with the fenestrated surgical drape. Lavage the incision site with sterile saline to clean the site and to loosen the sutures. Use a 70% ethanol soaked cotton swab to gently sterilize the