Why does null hypothesis testing matter for bacterial enumeration?

Null hypothesis testing in bacterial enumeration enables objective assessment of intervention effects by comparing CFU counts across experimental groups, supporting target validation and mechanistic clarity in infection models.

How does independent variable isolation fit the co-infection workflow?

Isolating variables such as pathogen type or treatment condition ensures that changes in bacterial load reflect specific experimental manipulations, strengthening discovery-stage conclusions and reducing confounding factors.

What do quantitative CFU measurements enable in preclinical studies?

Quantitative CFU measurements provide precise readouts of bacterial burden, enabling comparison of disease severity, intervention efficacy, and supporting data-driven advancement decisions in preclinical pipelines.

Why are replication requirements critical for cross-functional teams?

Replication of tissue processing and enumeration ensures data reliability, facilitates cross-team comparisons, and underpins collaborative decision-making in multi-disciplinary R&D environments.

Which statistical analysis capabilities are needed before implementing CFU quantification?

Robust statistical analysis of CFU data, including variance assessment and group comparisons, is essential to validate findings and support actionable insights in biopharma research workflows.

Processing of Infected Tissues for Bacterial Enumeration in a Mouse Co-Infection Model

JoVE Encyclopedia of Experiments

Microbiology

0 views • 3:16 min • March 31st, 2026

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

Begin with a mouse co-infected with a pathogenic bacterium and a virus.

The virus damages the epithelial lining of the mouse’s upper respiratory tract, facilitating the spread of bacteria from the nasopharynx to the lungs.

Place the harvested mouse lungs into a dish containing phosphate-buffered saline, or PBS.

Mince the lungs into small pieces and mix well.

Transfer half of the lung tissue into a tube containing PBS.

Place a sterilized tissue homogenizer probe inside the tube containing the lung tissue.

Homogenize the tissue to release the bacteria into the PBS.

Dilute the homogenate and plate the dilutions on a nutrient-rich agar plate.

Incubate the plate to allow bacterial colonies to form.

Count these colonies to quantify the lung bacterial load, which reflects bacterial burden in the lungs.

For lung collection, using dissection scissors, cut the sides of the exposed rib cage and gently pull the ribs up toward the head of the mouse to expose the heart. Insert a 25-gauge needle attached to a 10 milliliter syringe prefilled with PBS into the right ventricle and begin slowly perfusing. Look for bleaching of the lungs as an indicator of successful perfusion.

Flush slowly to avoid breaking the pulmonary tissue. Lift the heart with forceps and make a cut to separate the lungs and heart. Once separated, pick up all lobes of the lung with forceps and rinse in a dish with sterile PBS to remove any residual blood.

In a Petri dish, mince the lung into small pieces and mix well. Remove half of the lung mix to determine the bacterial CFU or viral PFU and place it in a round-bottom 15 milliliter tube prefilled with 0.5 milliliters of PBS for homogenization.

Related Videos

0 Views

Last updated: 1 August 2026

Processing of Infected Tissues for Bacterial Enumeration in a Mouse Co-Infection Model

Overzicht

Belangrijke studiecomponenten

Wetenschapsgebied

Achtergrond

Doel van de studie

Gebruikte methoden

Belangrijkste resultaten

Conclusies

Veelgestelde vragen

Executive Industry Relevance

Strategic Applications in Biopharma R&D

Early Discovery & Target Validation

Screening & Assay Development

Translational & Preclinical Research

Pipeline & Workflow Integration

Operational & Enterprise Impact

Implementation Considerations

Biopharma FAQs

Bestuderen van microbiële gemeenschappen In Vivo: Een model van de Host-gemedieerde interactie tussen Candida albicans En Pseudomonas aeruginosa In het Airways

De oprichting van een Long kolonisatie Assay voor het circulerende Tumor visualisatie van de cel in de weefsels van de longen

P. aeruginosa Geïnfecteerde 3D Co-Cultuur van Bronchiale epitheelcellen en macrofagen bij Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

Quantification of Tissue Bacterial Load in a Mouse Model of Chronic Intestinal Infection

Assessing Bacterial Burden in the Mouse Spleen for Infection Studies

Quantification of Bacterial Load in Mouse Spleen and Liver After Systemic Infection

De Citrobacter rodentium Muis Model: Studeren pathogeen en gastheer Bijdragen aan infectieuze colitis

4D multimodale beeldvorming van Citrobacter rodentium Infecties in Muizen

Gehumaniseerd muismodel om te studeren Bacteriële infecties Targeting microvasculature

Experimentele infectie met Listeria monocytogenes Als een model voor de studie Host Interferon-γ Responses