Blue Light Therapy for Drug-Resistant Bacterial Infections in Burn Wounds in a Mouse Model

Begin with an anesthetized mouse with its back exposed, placed on a Petri dish lid.

The mouse has a burn wound infected with bioluminescent, drug-resistant bacteria.

Cover the mouse’s eyes with aluminum foil to avoid overexposure to blue light.

Place the mouse under a blue LED device at a fixed distance.

Irradiate the wound at a constant intensity. Increase the exposure time to deliver higher doses.

The light penetrates the wound tissue and reaches the colonizing bacteria.

Within these bacteria, light-sensitive molecules absorb the blue light and become photoactivated, producing reactive oxygen species (ROS).

ROS damage bacterial components, including bioluminescent proteins, leading to bacterial cell death with minimal harm to the surrounding tissue.

After each exposure, perform bioluminescence imaging to monitor bacterial viability around the wound.

As the dose increases, the signal declines correspondingly.

This decline indicates the antibacterial effect of blue light-induced oxidative stress.

After anesthetizing the mice, according to the text protocol, randomly divide the animals into an antimicrobial blue light or aBL-treated group and an untreated control group. For the aBL-treated gro