JoVE Encyclopedie van Experimenten
Microbiologie
0 weergaven • 4:35 min. • February 26th, 2026
Begin with a microfluidic device mounted on a microscope stage.
The device comprises a polymer-glass assembly enclosing a microchannel with cylindrical pillars that mimic a porous medium.
A syringe pump delivers culture medium through a filter-fitted syringe into the channel, which drains via an outlet tubing into a waste container.
Monitor the baseline fluid pressure using a sensor connected to the channel.
Place the outlet tubing into a vial containing a bacterial suspension.
Reverse the flow until the suspension fills the channel, while the filter prevents bacteria from entering the syringe.
Incubate to allow the bacterial cells to adhere to surfaces, proliferate, and initiate biofilm formation.
Then, remove the filter and return the outlet to the waste container.
Resume medium flow and monitor the pressure as the biofilm grows and blocks the channel.
Capture time-lapse microscopic images to visualize biofilm growth over time. Correlate biofilm growth with pressure shifts to study bioclogging in porous media.
Begin by turning on the box incubator of the microscope three hours before the experiment to ensure a stable temperature of 25 degrees Celsius.
Connect the inlet and outlet tubing to
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