Spinal Cord Extraction: A Protocol to Isolate Murine Spinal Cord for In Vitro Studies

JoVE Encyclopedia of Experiments

Kankeronderzoek

0 weergaven • 3:08 min • April 30th, 2023

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

For spinal cord extraction, begin with a euthanized mouse in the prone position. Spray ethanol to prevent any potential contamination from fur.

Cut through the midline from the head to the tail and open the skin to reveal the underlying tissue. Examine the mouse’s skull to locate the craniocervical junction that connects the skull to the cervical spine.

Next, incise the craniocervical junction perpendicularly to sever the connection between the head and neck. Dissect the spine till the fifth lumbar vertebra. Subsequently, cut the spine at the fifth lumbar vertebra to sever the connection from the lower vertebral levels.

Now, turn the mouse to the supine position. Slit the peritoneum and the chest wall to expose both the abdominal and thoracic cavity. Remove the peritoneal and retroperitoneal organs to access the vertebral column.

Cut the ribs to separate the vertebral column from the rest of the body. Last, rinse the isolated spinal cord with a cold saline solution to wash away any blood contaminants.

After euthanizing the animal in a CO2 chamber, soak the fur with 70% ethanol and allow it to dry. Pin the mouse in a prone position and then make a midline incision extending craniocaudally from the posterior neck to the lumbar spine.

Next, use forceps to raise the dermal flaps bilaterally and expose the subcutaneous tissue. Palpate the mouse skull until the craniocervical junction is identified. Use scissors angled perpendicular to the animal to cut through the cervical muscles and spine at the craniocervical junction. Then, dissect the spine caudally and use scissors to perform a complete caudal transection at the fifth lumbar vertebrae.

Next, position the mouse in a supine position and, after making an incision along the midline from the neck to the abdomen, retract the skin laterally and then cut open the peritoneum.

Cranially, open the chest wall with scissors. After removing the peritoneal and retroperitoneal organs, use forceps and a surgical blade to cut the ribs, leaving approximately 5 millimeters of ribs protruding from the vertebral column. Remove the vertebral column from the rest of the body and wash twice with cold PBS.

09:10

De Voorbereiding van de Oblique Spinal Cord Slices voor Ventral Root Stimulation

Gerelateerde video's

0 Bekeken

08:10

Hydraulische extrusie van het ruggenmerg en isolatie van achterwortelganglia in Knaagdieren

Gerelateerde video's

0 Bekeken

07:49

Spinal Cord Neurons Isolatie en Cultuur van Neonatale Muizen

Gerelateerde video's

0 Bekeken

04:59

Spinal Cord Elektrofysiologie

Gerelateerde video's

0 Bekeken

06:10

Spinal Cord Neuron Isolation: A Density Gradient Procedure to Isolate Neonatal Spinal Cord Neurons for In Vitro Studies

Gerelateerde video's

0 Bekeken

04:30

Isolation of Neural Stem Progenitor Cells from the Spinal Cord Periventricular Region of an Adult Rat

Gerelateerde video's

0 Bekeken

11:18

Een Ex Vivo Laser-induced Spinal Cord Injury Model Mechanismen van axonale degeneratie Beoordeel in real-time

Gerelateerde video's

0 Bekeken

10:44

Twee-foton Imaging van Cellular Dynamics in de Muis Spinal Cord

Gerelateerde video's

0 Bekeken

08:26

Isolatie van neurale stamcellen / stamcellen uit de periventriculaire regio van de volwassen rat en Human Spinal Cord

Gerelateerde video's

0 Bekeken

09:08

Een test van de Neurosphere voor de evaluatie van de activering van de endogene neurale stamcellen in een muismodel van minimale dwarslaesie

Gerelateerde video's

0 Bekeken

Laatst bijgewerkt: 22 augustus 2026