A Technique to Generate a Murine Model of Dextran Sulfate Sodium-Induced Colitis

JoVE Encyclopedia of Experiments

Immunologie

0 weergaven • 3:20 min • July 8th, 2025

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

To induce colitis — an inflammatory bowel disease — in a murine model, take a mouse housed in a cage.

Replace the drinking water with a solution of dextran sulfate sodium, DSS, a colitis-inducing polysaccharide. Allow the mouse to consume the solution to develop colitis.

Upon consumption, DSS enters the lumen of the mouse's colon. The colon contains a thick mucus layer, providing a physical barrier between the commensal microbes and the underlying epithelial cell monolayer.

DSS penetrates the mucus layer, entering the epithelial cells. Inside the cells, DSS alters the expression of tight-junction proteins and induces apoptosis, resulting in the disruption of the epithelial layer and mucosal barrier. This allows the entry of luminal microbes into the lamina propria — the underlying tissue with resident dendritic cells and macrophages.

The immune cells recognize the microbes, activating downstream signaling to secrete pro-inflammatory cytokines. Cytokines cause the infiltration of a large number of T cells which mount an exaggerated immune response, resulting in inflammation.

Monitor the treated mouse for gastrointestinal disorders and weight loss, indicating the onset of colitis.

Euthanize the mouse; surgically isolate the entire colon. Divide the colon into proximal, middle, and distal sections and add a fixative for tissue preservation.

The tissue is ready for downstream assays to evaluate colitis.

To induce dextran sulfate sodium, or DSS colitis, replace the regular drinking water with 3% DSS solution ad libitum for seven days, measuring each mouse's body weight, DSS solution consumption, and stool production daily. At the end of the seven-day treatment, place the mouse in the supine position, and use small scissors to make a three-centimeter-long midline incision in the abdomen.

Then, use forceps to lift the colon to allow it to be separated from the surrounding mesentery, and extract the whole colon until the cecum and rectum are visible. Transect the tissue at the colonocecal margin and deep within the pelvis, to free the proximal and distal colon respectively.

Measure and record the length of the isolated colon, and use a 10-milliliter syringe equipped with a gavage needle to flush the colon with 10 milliliters of ice-cold PBS to remove the feces and blood until the eluate runs clear. For histological identification, divide the tissue samples into equally-sized proximal, middle, and distal sections, and fix the tissues in 4% paraformaldehyde overnight at four degrees Celsius.

09:11

Systematische scoringsanalyse voor darmontsteking in een muriene dextran natriumsulfaat-geïnduceerde colitismodel

Gerelateerde video's

0 Bekeken

07:34

Inductie van darmontsteking door adoptieve overdracht van CBir1 TCR transgene CD4 + T-cellen naar immunodeficiënte muizen

Gerelateerde video's

0 Bekeken

05:08

Een TNBS-geïnduceerd knaagdiermodel om de pathogene rol van mechanische stress bij de ziekte van Crohn te bestuderen

Gerelateerde video's

0 Bekeken

08:39

Murine Colitis Modeling met dextraansulfaat Natrium (DSS)

Gerelateerde video's

0 Bekeken

08:43

Onderzoeken darmontsteking in DSS-geïnduceerde Model van IBD

Gerelateerde video's

0 Bekeken

12:37

Modeling Colitis geassocieerde kanker met Azoxymethaan (AOM) en dextransulfaat natrium (DSS)

Gerelateerde video's

0 Bekeken

03:50

Hematoxylin and Eosin Staining to Assess Colon Damage in DSS-Induced Colitis

Gerelateerde video's

0 Bekeken

03:23

Assessment of Chemical-Induced Colitis via Immunohistochemical Analysis of Colon Tissue Sections

Gerelateerde video's

0 Bekeken

09:04

DNBS / TNBS Colitis modellen: Inzicht geven Into Inflammatory Bowel Disease en effecten van vet in de voeding

Gerelateerde video's

0 Bekeken

08:20

Lymfkliertest distale colostoma, een nieuw Model van afleiding Colitis in C57BL/6 muizen

Gerelateerde video's

0 Bekeken

Laatst bijgewerkt: 15 augustus 2026