JoVE Encyclopedie van Experimenten
Neurowetenschap
0 weergaven • 3:06 min. • July 8th, 2025
Begin by adding neuronal cells to a suitable medium in a culture dish.
Add fluorescent magnetic nanoparticles, or MNPs, containing a magnetic core tagged with a fluorescent dye.
During incubation, the cell takes up the MNP through endocytosis and becomes magnetized.
Place a clean magnetized patterned substrate in a culture dish. This glass substrate has embedded geometrical patterns with magnetic properties.
Sterilize the substrate by exposing it to ultraviolet light.
Coat the substrate with collagen to promote cell attachment.
Add the MNP-loaded cells to the substrate and incubate.
The external magnetic field of the substrate attracts the MNP-loaded cells, enabling their attachment.
Add a medium containing a specific growth factor at regular intervals.
The growth factor assists cells in forming extensions, while the magnetic pattern of the substrate directs the orientation of these extensions.
Seed 1 million cells in a regular, uncoated 35-millimeter dish. Add the calculated volume of MNP suspension and differentiation medium to the dish to achieve the desired MNP concentration. Mix the cells, MNPs, and differentiation medium, then incubate the dish in a 5% carbon dioxide humidifi
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