Constructing Patterned Neuronal Circuits on a Multi-Electrode Array

Take a cleaned and sterilized multi-electrode array, or MEA, a device containing microelectrodes to obtain and deliver neural signals.

Attach a patterned polymeric stencil to a micromanipulator and place it on the MEA surface.

Under a microscope, align the patterned stencil with the MEA's electrodes and attach it to create modules.

Remove the micromanipulator.

Transfer the MEA to a vacuum chamber. Add a matrix solution to the stencil and apply a vacuum to ensure an even matrix coating.

Allow the matrix to dry.

Remove the stencil, and sterilize the MEA by exposing it to ultraviolet light.

Add neuronal cells to the center of the MEA and allow the cells to attach to the matrix layer.

Next, add growth media and incubate.

Nutrients from the media and the extracellular matrix enable the cells to grow and extend projections, forming interconnected patterned neuronal circuits whose signals can be recorded using the MEA.

First, clean the multielectrode array by washing it under tap water, and then sonicating it in a concentrated enzymatic detergent. Repeat these steps three times. Then, sonicate the MEA in pure distilled water three times. After which, under a hood, wash the MEA with distil