Patch-Clamp Recordings from the Dendrite of a Dopaminergic Neuron in a Brain Slice

Begin with an electrophysiology setup with an immobilized brain slice submerged in an artificial cerebrospinal fluid or aCSF containing different ions.

The setup includes a patch pipette filled with a potassium-rich solution connected to a recording electrode and an amplifier.

Apply positive air pressure to the pipette to prevent its clogging. Then, position it near a dendrite, the signal-receiving extension of a dopaminergic neuron.

The resulting membrane dimpling confirms proximity to the dendrite.

Release the positive pressure to create suction, forming a tight seal between the pipette and the dendritic membrane.

Record the initial ion movement as an action current at the baseline voltage.

Flow-in aCSF with sodium and calcium channel blockers to block the respective channels, preventing ion movement through them.

Apply a negative voltage to open a voltage-gated cation channel.

This channel allows potassium ions to enter the neuron, increasing the action current and suggesting stimulation of the dendrite in a dopaminergic neuron.

In this procedure, transfer a brain slice to the recording chamber. Anchor the slice at the bottom of the recording chamber with a platinum ring, and en