Utilizing Multielectrode Arrays to Measure Synaptic Physiology in 3D Coculture Spheres

JoVE Encyclopedia of Experiments

Neurowetenschap

0 weergaven • 2:07 min • July 8th, 2025

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

Take a sterile multielectrode array or MEA, a device that records the electrical activity of target cells.

Add a polymer solution to the MEA and incubate.

The polymer coats the surface of the MEA, thereby increasing the water-attracting property of the surface.

Remove the excess polymer and wash with deionized water.

Add extracellular matrix, or ECM, and incubate to allow it to coat the MEA. Remove the excess ECM.

Next, add the 3D coculture spheres comprising neurons and astrocytes in a suitable media.

Incubate to allow the spheres to adhere to the surface of the MEA.

Place the MEA on a temperature-controlled head stage of an MEA system and record the spontaneous electrical activity across the electrodes.

The measured electrical activity corresponds to the communication at the neural junctions within the sphere.

Begin by coating the surface of each multielectrode array or MEA with 1 millimeter of poly-ornithine to render the surface hydrophilic and incubate at 37 degrees Celsius for a minimum of four hours. Following the incubation, remove the poly-ornithine and wash the surface of each MEA with deionized water. Then, add 1 milliliter of extracellular matrix and return to the incubator for a minimum of four hours.

When ready, remove the extracellular matrix, then apply the spheres in 1.5 milliliters of medium onto the MEA surface, ensuring that the spheres are positioned on top of the electrode array. Allow to adhere for two days. To measure the electrical activity of neural spheres, place the MEA on a temperature-controlled head stage.

09:39

Direct-current stimulatie en multi-elektrode Array Registratie van Inbeslagneming-achtige activiteit in de muizen Brain Slice Voorbereiding

Gerelateerde video's

0 Bekeken

09:58

Fabricage van Ti3C2 MXene Microelectrode Arrays voor In Vivo Neural Recording

Gerelateerde video's

0 Bekeken

11:28

Netwerkactiviteit opnemen in spinale nociceptieve circuits met behulp van micro-elektrodearrays

Gerelateerde video's

0 Bekeken

09:27

Hoe te Cultuur, Record en het stimuleren van neuronale netwerken op de Micro-elektrode arrays (MEA's)

Gerelateerde video's

0 Bekeken

16:01

Multi-electrode array Opnamen van neuronale Lawines in organotypische culturen

Gerelateerde video's

0 Bekeken

02:39

Building Three-Dimensional Neuronal Networks Coupled to Micro-Electrode Arrays

Gerelateerde video's

0 Bekeken

02:35

Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays

Gerelateerde video's

0 Bekeken

02:26

Generating 3D Co-culture Spheres of Astrocytes and Neurons to Induce Synapse Formation

Gerelateerde video's

0 Bekeken

02:05

Measuring the Electrophysiological Activity of Neuronal Networks Using Micro-Electrode Arrays

Gerelateerde video's

0 Bekeken

09:47

Interfacing 3D Engineered neuronale culturen op micro-elektrode arrays: een innovatieve In Vitro Experimental Model

Gerelateerde video's

0 Bekeken

Laatst bijgewerkt: 15 augustus 2026