Monitoring Seizure-Induced Neural Electrical Activity in Brain Slices Using Microelectrode Arrays

Begin by placing a brain slice on a microelectrodearray or MEA recording chamber filled with artificial cerebrospinal fluid or aCSF containing the test drug.

Adjust the position of the slice and secure it with an anchor.

Start the continuous flow of the aCSF, containing a seizure-inducing test drug.

Capture an image of the slice.

Select the appropriate microelectrodes in contact with the brain slice.

Activate the stimulus unit and connect the wires to the MEA unit.

Record the baseline signals.

Perform a test run to determine the optimal stimulus intensity.

Now, start delivering electrical pulses.

These pulses stimulate the selected brain region, inducing an artificial seizure.

Record the resulting electrical activity to capture seizure-evoked responses.

Once the 4-AP aCSF level and the recording temperature are stabilized as desired, turn the perfusion and the suction stopcocks to the off position to temporarily stop them. Quickly transfer one brain slice onto the MEA recording chamber using an inverted glass Pasteur pipette.

Adjust its position on the MEA recording area as needed, using a fire-polished curled Pasteur pipette or a soft, compact small brush. Place the hold-down anch