Establishing a Subarachnoid Hemorrhage Rat Model Induced by Autologous Blood Injection

Take an anesthetized rat with its skull exposed and a probe implanted to measure intracranial pressure.

Using a reference point on the skull, mark the target site and drill a hole.

Remove the bone fragments and fill the hole with bone wax.

Drill another hole. Locate a blood vessel and secure a probe to measure cerebral blood flow.

Take a needle and insert it into the brain until the skull is felt.

Retract slightly to ensure its placement in front of the optic chiasm, where major blood vessels are located.

Fill the needle chamber with fresh blood drawn from the rat's tail and connect the syringe containing blood.

Inject the blood into the fluid-filled subarachnoid space between the outer and inner brain membranes, which increases intracranial pressure, causing blood vessel constriction.

This leads to a decreased cerebral blood flow, establishing a subarachnoid hemorrhage model.

To place the laser Doppler probe, make a 15-millimeter incision caudally in the midline, starting just anterior to the eyes. After removing the connective tissue and the muscles with forceps, use the end of a sterile cotton swab as a regime to identify the bregma and the coronal sutures.

After placing the arm