Evaluating the Effect of Continuous Drug Infusion into the Mouse Brain on Peripheral Glucose Levels

Begin with a mouse implanted with a micro-osmotic pump, which continuously delivers a drug directly into the brain over a set period.

This drug is an antagonist of a chemokine that binds to its receptor, blocking hypothalamic insulin signaling and impairing glucose metabolism.

Fast the mouse for the desired time interval.

Pick up the mouse and stroke the tail to ensure sufficient blood flow.

Cut off a small portion of the tail to obtain a drop of blood.

Place the blood drop onto the glucose chip inserted into a glucometer and record the glucose level.

Then, feed the mouse a glucose solution orally.

Measure the glucose levels at desired time intervals.

Persistently high glucose levels show impaired glucose metabolism by the drug.

Before starting the glucose tolerance test, prepare the glucose solution by dissolving 3.75 grams glucose and 15 milliliters distilled water. Set up a time table with proper intervals between each blood examination to record the readings during the experiment. Next, weigh each mouse after fasting to calculate the appropriate amount of glucose for the injection. Then at the workbench, prepare timer, glucose chip, glucometer, insulin syringe, and razor blades