Generating a Glioblastoma Rat Model

Take an anesthetized rat.

Shave the scalp. Then, apply eye gel to maintain corneal hydration.

Secure the animal on a stereotactic frame and disinfect the surgical site. Make a midline incision and retract it to expose the skull.

Drill a hole in the right frontal hemisphere to access the prefrontal cortex.

Position a stereotactically guided syringe containing a suspension of glioblastoma, or brain tumor, cells over the drilled site.

Using a pump controller, inject the cell suspension into the prefrontal cortex.

Slowly withdraw the needle to prevent reflux of the injected cells. Then, seal the drilled site with bone wax.

Suture the surgical incision and disinfect the site.

Return the rat to its cage and use a red lamp to maintain body temperature.

Following injection, the brain tumor cells proliferate, infiltrate surrounding tissue, and induce angiogenesis, disrupting normal brain function and generating a glioblastoma model.

To inoculate the brain of an anesthetized 170 gram, female, fisher F344 rat with glioma cells, first, confirm sedation by lack of response to toe pinch. Remove the hair from eye-level to the back of the skull, and apply ointment to the animals eyes. Immobilize the anim