Transplantation of Interneuron Precursor Cells into the Brain of a Mouse Pup

Begin with an anesthetized recipient mouse pup. Secure its head with tape, ensuring that the skull remains exposed.

Use the anatomical reference lambda to locate the target brain region.

Position a micropipette over the target region, loaded with a suspension of interneuron precursor cells capable of differentiating into mature interneurons.

These precursor cells are derived from the cortex, striatum, or hippocampus of a donor pup at the same developmental stage as the recipient.

Inject the suspension into the brain to perform either a homotopic transplant, in which precursors are delivered to their region of origin, or a heterotopic transplant, where they are introduced into a different brain region.

Allow the suspension to diffuse before retracting the micropipette to prevent backflow.

Transfer the pup onto a warming pad for recovery.

The survival and maturation of the donor precursors in the brain of the recipient pup can now be evaluated.

Load a fine-tipped micropipette with mineral oil. Attach the micropipette to a nanoliter injector, and secure the nanoliter injection apparatus to a manipulator attached to a magnetic base. While the first pup is being anesthetized on ice, pipette th