Name: Analyse van menselijke T-celactiviteit in een allogene co-cultuursetting van voorbehandelde tumorcellen (Video) | JoVE Journal
Uploaded: 2025-03-07T13:40:00.000+00:00
Duration: 9 min 4 s
Description: Leer een co-cultuurmethode voor T-celstimulatie met voorbehandelde tumorcellen. Dit protocol analyseert menselijke T-celactiviteit na radiotherapie en kinase-inhibitoren.

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analyse van menselijke t-celactiviteit in een allogene co-cultuursetting van voorbehandelde tumorcellen

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Analyse van menselijke T-celactiviteit in een allogene co-cultuursetting van voorbehandelde tumorcellen

Cytotoxic T cells play a key role in the elimination of tumor cells and are, therefore, intensively studied in cancer immunology. The frequency and ...

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Dit protocol beschrijft de chirurgische generatie van orthotopic alvleesklier tumoren en de snelle vertering van vers geïsoleerde lymfatische alvleesklier tumoren. Na de spijsvertering kunnen levensvatbare immuuncelpopulaties worden gebruikt voor verdere downstream-analyse, inclusief ex-vivo stimulatie van T-cellen voor intracellulaire cytokine detectie door Flowcytometrie.

Generatie van Orthotopic alvleesklier tumoren en ex vivo karakterisering van tumor-Infiltrerende T cel cytotoxiciteit

Hier beschrijven we een eenvoudig, snel en betrouwbaar multiparametrisch flowcytometrie- en tumor-op-een-chip-ondersteund protocol om de stappen die de kankerimmuniteitscyclus vormen, te bewaken en te karakteriseren. Een grondig begrip van de wisselwerking tussen kanker en immuuncellen biedt inderdaad cruciale inzichten om tumoren te slim af te zijn en de klinische zorg te sturen.

Co-cultuur in vitro-systemen om de kanker-immuniteitscyclus te reproduceren

Leer een co-cultuurmethode voor T-celstimulatie met voorbehandelde tumorcellen. Dit protocol analyseert menselijke T-celactiviteit na radiotherapie en kinase-inhibitoren.

Analyse van menselijke T-celactiviteit in een allogene co-cultuursetting van voorbehandelde tumorcellen

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