Name: Gewijzigde Most Probable Number Assay om salmonella in rauwe en kant-en-klare kipproducten te kwantificeren (Video) | JoVE Journal
Uploaded: 2025-01-31T14:55:00.000+00:00
Duration: 8 min 19 s
Description: Leer een gewijzigde MPN-assay om Salmonella in rauwe en kant-en-klare kipproducten te kwantificeren. Deze methode verbetert voedselveiligheid door snelle detectie met FSIS-protocollen.

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<table><tbody><tr><td>48 deep well block 4.6ml</td><td>Fisher Scientific International, Inc</td><td>NC1964628</td><td></td></tr><tr><td>Agar - Solidifying Agent (Difco)</td><td>Becton, Dickinson and Company (BD)</td><td>281230</td><td></td></tr><tr><td>Analytical Balance</td><td>Mettler Toledo</td><td>JL602-G/L</td><td>Equipment&nbsp;</td></tr><tr><td>Analytical Balance</td><td>Mettler Toledo</td><td>AB54-S</td><td>Equipment&nbsp;</td></tr><tr><td>Autoclave - Amsco Lab250, Laboratory Steam Sterilizer</td><td>Steris plc</td><td>LV-250</td><td>Equipment&nbsp;</td></tr><tr><td>Biological Safety Cabinet, Type A2, Purifier Logic+</td><td>Labconco Corporation</td><td>302411101</td><td>Equipment&nbsp;</td></tr><tr><td>Brain Heart Infusion (BHI) Broth</td><td>Becton, Dickinson and Company (BD)</td><td>237500</td><td></td></tr><tr><td>Buffered Peptone Water</td><td>Bio-Rad Laboratories Inc.</td><td>3564684</td><td></td></tr><tr><td>Cell Spreader - L-shaped</td><td>VWR</td><td>76208-438</td><td></td></tr><tr><td>Centrifuge Microcentrifuge 5424</td><td>Eppendorf</td><td>5424</td><td>Equipment</td></tr><tr><td>Centrifuge, Avanti J-25</td><td>Beckman Coulter, Inc.&nbsp;</td><td></td><td>Equipment</td></tr><tr><td>DNA Extraction - PreMan Ultra Sample Preparation Reagent&nbsp;</td><td>Thermo Fisher Scientific Inc.&nbsp;</td><td>4318930</td><td></td></tr><tr><td>Ground Chicken&nbsp;</td><td>Local retailers</td><td></td><td></td></tr><tr><td>IAC forward&nbsp; primer: 5'-GGCGCGCCTAACACATCT-3'</td><td>Integrated DNA Technologies&nbsp;</td><td></td><td></td></tr><tr><td>IAC probe: 5'-TTACAACGGGAGAAGACAATGC CACCA-3' labeled with 5' TAMRA/3' BHQ-2</td><td>Biosearch Technologies</td><td></td><td></td></tr><tr><td>IAC reverse primer: 5'-TGGAAGCAATGCCAAATGTGTA-3'</td><td>Integrated DNA Technologies&nbsp;</td><td></td><td></td></tr><tr><td>Incubator - Inova 4230 incubator shaker</td><td>New Brunswick Scientific</td><td>4230</td><td>Equipment&nbsp;</td></tr><tr><td>Inoculating Loop - Combi Loop&nbsp; 10&micro;L and 1&micro;L&nbsp;</td><td>Fisher Scientific International, Inc</td><td>22-363-602</td><td></td></tr><tr><td>invA forward primer: 5'-GTTGAGGATGTTATTCGCAAAG G-3'</td><td>Integrated DNA Technologies&nbsp;</td><td></td><td></td></tr><tr><td>invA probe: 5'-CCGTCAGACCTCTGGCAGTAC CTTCCTC-3' labeled with 5' Cal Fluor Orange 560/3' BHQ-1</td><td>Biosearch Technologies</td><td></td><td></td></tr><tr><td>invA reverse primer: 5'-GGAGGCTTCCGGGTCAAG-3'</td><td>Integrated DNA Technologies&nbsp;</td><td></td><td></td></tr><tr><td>Irradiation Treatment</td><td>Texas A&amp;M Agrilife Research National Center for Electron Beam Research</td><td></td><td>Service</td></tr><tr><td>Luria Bertani (LB) Broth</td><td>Becton, Dickinson and Company (BD)</td><td>244620</td><td></td></tr><tr><td>Manual pipette Pipet-Lite LTS Pipette L-1000XLS+</td><td>Mettler Toledo</td><td>17014382</td><td>Equipment</td></tr><tr><td>Manual pipette Pipet-Lite LTS Pipette L-100XLS+</td><td>Mettler Toledo</td><td>17014384</td><td>Equipment</td></tr><tr><td>Manual pipette Pipet-Lite LTS Pipette L-10XLS+</td><td>Mettler Toledo</td><td>17014388</td><td>Equipment</td></tr><tr><td>Manual pipette Pipet-Lite LTS Pipette L-200XLS+</td><td>Mettler Toledo</td><td>17014391</td><td>Equipment</td></tr><tr><td>Manual pipette Pipet-Lite LTS Pipette L-20XLS+</td><td>Mettler Toledo</td><td>17014392</td><td>Equipment</td></tr><tr><td>Manual pipette Pipet-Lite Multi Pipette L8-200XLS+</td><td>Mettler Toledo</td><td>17013805</td><td>Equipment</td></tr><tr><td>Manual pipette Pipet-Lite Multi Pipette L8-20XLS+</td><td>Mettler Toledo</td><td>17013803</td><td>Equipment</td></tr><tr><td>Media Storage Bottle -PYREX 1L Square Glass&nbsp; Bottle, with GL45 Screw Cap</td><td>Corning Inc.</td><td>1396-1L</td><td>Equipment</td></tr><tr><td>Media Storage Bottle -PYREX 2L Round Wide Mouth Bottle, with GLS80 Screw Cap</td><td>Corning Inc.</td><td>1397-2L</td><td>Equipment</td></tr><tr><td>Microtiter plate, 96 well plate, flat bottom, polystyrene, 0.34cm2, sterile, 108/cs</td><td>MilliporeSigma</td><td>Z707902</td><td></td></tr><tr><td>Mixer - Vortex Genie 2</td><td>Scientific Industries Inc.</td><td>SI-0236</td><td>Equipment</td></tr><tr><td>Molecular Detection Assay 2-Salmonella kit</td><td>Neogen</td><td>MDA2SAL96</td><td></td></tr><tr><td>Molecular Detection Instrument&nbsp;</td><td>Neogen</td><td>MDS100</td><td>Equipment&nbsp;</td></tr><tr><td>Motorized pipette controller, PIPETBOY2</td><td>INTEGRA Biosciences Corp.</td><td>155019</td><td>Equipment</td></tr><tr><td>PCR Mastermix 2&times; TaqMan Gene Expression&nbsp;</td><td>Thermo Fisher Scientific Inc.&nbsp;</td><td>4369542</td><td></td></tr><tr><td>Petri Dish Rotator -&nbsp; bioWORLD Inoculation Turntable</td><td>Fisher Scientific International, Inc</td><td>3489E20</td><td>Equipment</td></tr><tr><td>Petri Dishes with Clear Lid (100 mm x 15mm)</td><td>Fisher Scientific International, Inc</td><td>FB0875713</td><td></td></tr><tr><td>Pipette Tips GP LTS 1000&micro;L S 768A/8</td><td>Mettler Toledo</td><td>&nbsp;30389273</td><td></td></tr><tr><td>Pipette Tips GP LTS 20&micro;L 960A/10</td><td>Mettler Toledo</td><td>30389270</td><td></td></tr><tr><td>Pipette Tips GP LTS 200&micro;L F 960A/10</td><td>Mettler Toledo</td><td>30389276</td><td></td></tr><tr><td>Ready to cook chicken products</td><td>Local retailers</td><td></td><td></td></tr><tr><td>Reagent Reservoir, 25 mL sterile reservoir used with multichannel pipettors</td><td>Thermo Fisher Scientific Inc.&nbsp;</td><td>8093-11</td><td></td></tr><tr><td>Realtime PCR - 7500 Real-Time PCR system&nbsp;</td><td>(Applied Biosystems, Foster City, CA)</td><td>2750036476</td><td>Equipment</td></tr><tr><td>Serological Pipettes, Nunc Serological Pipettes (10 mL)</td><td>Thermo Fisher Scientific Inc.&nbsp;</td><td>170356N</td><td></td></tr><tr><td>Serological Pipettes, Nunc Serological Pipettes (2 mL)</td><td>Thermo Fisher Scientific Inc.&nbsp;</td><td>170372N</td><td></td></tr><tr><td>Serological Pipettes, Nunc Serological Pipettes (25 mL)</td><td>Thermo Fisher Scientific Inc.&nbsp;</td><td>170357N</td><td></td></tr><tr><td>Serological Pipettes, Nunc Serological Pipettes (50 mL)</td><td>Thermo Fisher Scientific Inc.&nbsp;</td><td>170376N</td><td></td></tr><tr><td>Spreader - Fisherbrand L-Shaped Cell Spreaders</td><td>Fisher Scientific International, Inc</td><td>14-665-230</td><td></td></tr><tr><td>Stomacher bag, Nasco Whirl-Pak Write-On Homogenizer Blender Filter Bags</td><td>Thermo Fisher Scientific Inc.&nbsp;</td><td>01

gewijzigde most probable number assay om salmonella in rauwe en kant-en-klare kipproducten te kwantificeren

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Gewijzigde Most Probable Number Assay om salmonella in rauwe en kant-en-klare kipproducten te kwantificeren

Salmonella is a leading cause of foodborne illness in the United States, particularly in poultry products. Traditional methods for detecting Salmonella ...

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Hier introduceren we het principe, de structuur en de instructie van het intelligente high-throughput antimicrobiële gevoeligheidstest-/faagscreeningsysteem. De toepassing ervan wordt geïllustreerd aan de hand van Salmonella geïsoleerd uit pluimvee in Shandong, China, als voorbeeld. De Lar-index wordt berekend en de betekenis ervan bij het evalueren van antimicrobiële resistentie wordt uitgebreid besproken.

Toepassing van het intelligente high-throughput antimicrobiële gevoeligheidstest-/faagscreeningsysteem en Lar Index van antimicrobiële resistentie

Campylobacter is wereldwijd de belangrijkste oorzaak van bacteriële gastro-enteritis door voedsel. Ondanks het feit dat bedrijven maatregelen nemen om de prevalentie in hun faciliteiten te verminderen, bereiken besmette producten consequent de consument. De techniek die in de afgelopen twaalf jaar is ontwikkeld, richt zich op de beperkingen van bestaande methoden voor het isoleren en detecteren van Campylobacter spp. uit rauw vlees.

Detectie en isolatie van Campylobacter spp. uit rauw vlees

Leer een gewijzigde MPN-assay om Salmonella in rauwe en kant-en-klare kipproducten te kwantificeren. Deze methode verbetert voedselveiligheid door snelle detectie met FSIS-protocollen.

Gewijzigde Most Probable Number Assay om salmonella in rauwe en kant-en-klare kipproducten te kwantificeren

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