Name: Onderzoek naar interacties tussen myeloïde cellen en CAR T-cellen in vitro en in vivo (Video) | JoVE Journal
Uploaded: 2025-07-25T13:30:00.000+00:00
Duration: 5 min 14 s
Description: Leer vereenvoudigde in vitro en in vivo modellen om interacties tussen humane macrofagen en CAR T-cellen te bestuderen in mantelcellymfoom. Deze methoden ondersteunen het testen van macrofaag-gerichte immunotherapieën.

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5 minutes

<ol><li>Yun, K., Siegler, E. L., Kenderian, S. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Who+wins+the+combat%2C+CAR+or+TCR%5BTitle%5D)+AND+%22Leukemia%22%5BJournal%5D)">Who wins the combat, CAR or TCR.</a> Leukemia. 37 (10), 1953-1962 (2023).</li>
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<li>Sterner, R. M., Cox, M. J., Sakemura, R., Kenderian, S. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Using+crispr%2Fcas9+to+knock+out+gm-csf+in+car-t+cells%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Using crispr/cas9 to knock out gm-csf in car-t cells.</a> J Vis Exp. (149), e59629(2019).</li>
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<li>Ruella, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Overcoming+the+immunosuppressive+tumor+microenvironment+of+Hodgkin+lymphoma+using+chimeric+antigen+receptor+T+cells%5BTitle%5D)+AND+%22Cancer+Dis%22%5BJournal%5D)">Overcoming the immunosuppressive tumor microenvironment of Hodgkin lymphoma using chimeric antigen receptor T cells.</a> Cancer Dis. 7 (10), 1154-1167 (2017).</li>
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<li>Yun, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intrinsic+immunosuppressive+features+of+monocytes+suppress+CAR-T19+through+IL-1+pathway+modulation+in+mantle+cell+lymphoma%5BTitle%5D)+AND+%22Mol+Ther+Oncol%22%5BJournal%5D)">Intrinsic immunosuppressive features of monocytes suppress CAR-T19 through IL-1 pathway modulation in mantle cell lymphoma.</a> Mol Ther Oncol. 33 (2), 200985(2025).</li>
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onderzoek naar interacties tussen myeloïde cellen en car t-cellen in vitro en in vivo

Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo

Onderzoek naar interacties tussen myeloïde cellen en CAR T-cellen in vitro en in vivo

Chimeric antigen receptor T (CART) cell therapy has led to remarkable clinical successes treating hematological malignancies. However, most patients ...

studying-interactions-between-myeloid-cells-car-t-cells-vitro

Research

JoVE Journal

Cancer Research

Kankeronderzoek

This video demonstrates an in vitro assay to validate chimeric antigen receptor (CAR) T cell activity. B-cell lymphoma cells expressing CD19 antigen and cytoplasmic luciferase are co-cultured with CD19-specific CAR T cells. CAR binding to CD19 on a tumor induces the CAR T cells to release cytokines and toxins that cause tumor cell cytotoxicity. The CAR T cell activity is evaluated by quantifying the released cytokine and decreased luciferase-mediated bioluminescence in the tumor cells.

An In Vitro Assay to Evaluate the Anti-Tumor Activity of Tumor-Specific CAR T Cells

Hier presenteren we een protocol voor de in vivo bepaling van naïeve CD4 T cel (T-cel) activering, proliferatie en differentiatie van de Th1 geïnduceerd door GM-CSF beenmerg (BM)-afgeleide dendritische cellen (DC's). Dit protocol beschrijft Daarnaast BM en T-cel isolatie, DC generatie en DC en T-cel adoptief overdracht.

Een muismodel In Vivo maatregel naïef CD4 T-cel activatie, proliferatie en differentiatie van de Th1 geïnduceerd door het beenmerg-afgeleide dendritische cellen

Hier presenteren we een protocol voor de productie en de preklinische beproeving van lymfkliertest CD19 auto T-cellen door retrovirale transductie en gebruik als een therapie tegen gevestigde syngeneic A20 B-cel lymfoom in BALB/c muizen met of zonder lymphodepleting Preconditionering.

Een Syngeneic B-cel lymfoom muismodel voor pre-klinische evaluatie van CD19 auto T cellen

Hier beschrijven we een in vitro co cultuur methode recursief uitdaging tumor-gerichte T-cellen, die het mogelijk voor fenotypische en functionele analyse van antitumorale T cel activiteit maakt.

In Vitro Tumor cel Rechallenge voor voorspellende evaluatie van chimeer antigeen Receptor T cel antitumorale functie

We beschrijven een aanpak om betrouwbaar te genereren chimeer antigeen receptor (auto) T cellen en testen hun differentiatie en functie in vitro en in vivo.

Productie van Chimerische antigeen receptor (auto) T cellen voor adoptie immunotherapie

Dit protocol stroomlijnt de productie van retrovirale vectoren en de transductie van T-cellen bij muizen, waardoor de efficiënte generatie van CAR-T-cellen van muizen wordt vergemakkelijkt.

Efficiënte generatie van muizen chimere antigeenreceptor (CAR)-T-cellen

Dit protocol biedt een methode voor primaire muizen T-celisolatie en time-lapse-microscopie van T-celmigratie onder specifieke omgevingsomstandigheden met kwantitatieve analyse.

Real-time in-vitromigratietest voor primaire muizen CD8+ T-cellen

Het huidige protocol beschrijft een experimentele workflow die de ex vivo analyse van menselijke T-celstimulatie mogelijk maakt in een allogene...

Analyse van menselijke T-celactiviteit in een allogene co-cultuursetting van voorbehandelde tumorcellen

Hier beschrijven we een methode voor het maken van dubbele emulsiedruppeltjes die één T-cel en een kankerdoelcel inkapselen om celdoding op eencellig niveau te onderzoeken. Deze methode maakt dubbele kwantificering mogelijk van zowel cytotoxische moleculen als apoptose van doelcellen binnen een grote populatie T-cellen.

Op druppels gebaseerde cytotoxiciteitstest om chimere antigeenreceptor-T-cellen op eencellig niveau te beoordelen

Dit protocol beschrijft de essentiële stappen voor de kweek van primaire T-cellen, waaronder hun isolatie, activatie, expansie en tijdelijke transfectie door mRNA-elektroporatie. Daarnaast worden een workflow en antilichaampanel voorgesteld voor flowcytometrische analyse van fenotype, activatiemarkers, levensvatbaarheid en chimere antigeenreceptor (CAR)-expressie.

Isolatie, cultivatie en tijdelijke transfectie van primaire menselijke T-cellen om chimerische antigeenreceptoren (CAR) T-cellen te genereren

Leer vereenvoudigde in vitro en in vivo modellen om interacties tussen humane macrofagen en CAR T-cellen te bestuderen in mantelcellymfoom. Deze methoden ondersteunen het testen van macrofaag-gerichte immunotherapieën.

Onderzoek naar interacties tussen myeloïde cellen en CAR T-cellen in vitro en in vivo

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