Name: Mitochondriaal preparaat uit microglia voor glycaananalyse (Video) | JoVE Journal
Uploaded: 2025-05-30T14:10:00.000+00:00
Duration: 6 min 40 s
Description: Leer hoe je mitochondria isoleert uit BV-2 microglia voor glycaananalyse met infrarood-MALDESI-massaspectrometrie. Deze methode helpt onderzoekers de rol van glycanen in neuro-immunologische interacties te begrijpen.

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5 minutes

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<li>Hickman, S., Izzy, S., Sen, P., Morsett, L., El Khoury, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microglia+in+neurodegeneration%5BTitle%5D)+AND+%22Nat+Neurosci%22%5BJournal%5D)">Microglia in neurodegeneration.</a> Nat Neurosci. 21 (10), 1359-1369 (2018).</li>
<li>Norat, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mitochondrial+dysfunction+in+neurological+disorders%3A+Exploring+mitochondrial+transplantation%5BTitle%5D)+AND+%22NPJ+Regen+Med%22%5BJournal%5D)">Mitochondrial dysfunction in neurological disorders: Exploring mitochondrial transplantation.</a> NPJ Regen Med. 5 (1), 22(2020).</li>
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<li>Fairley, L. H., Wong, J. H., Barron, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mitochondrial+regulation+of+microglial+immunometabolism+in+alzheimer%27s+disease%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Mitochondrial regulation of microglial immunometabolism in alzheimer's disease.</a> Front Immunol. 12, 624538(2021).</li>
<li>Starossom, S. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Galectin-1+deactivates+classically+activated+microglia+and+protects+from+inflammation-induced+neurodegeneration%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Galectin-1 deactivates classically activated microglia and protects from inflammation-induced neurodegeneration.</a> Immunity. 37 (2), 249-263 (2012).</li>
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<li>Gerlach, J. Q., Kilcoyne, M., Eaton, S., Bhavanandan, V., Joshi, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-carbohydrate-mediated+interaction+of+lectins+with+plant+proteins%5BTitle%5D)+AND+%22Adv+Exp+Med+Biol%22%5BJournal%5D)">Non-carbohydrate-mediated interaction of lectins with plant proteins.</a> Adv Exp Med Biol. 705, 257-269 (2011).</li>
<li>Cummings, R. D., Pierce, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+challenge+and+promise+of+glycomics%5BTitle%5D)+AND+%22Chem+Biol%22%5BJournal%5D)">The challenge and promise of glycomics.</a> Chem Biol. 21 (1), 1-15 (2014).</li>
<li>Robichaud, G., Barry, J. A., Muddiman, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((IR-MALDESI+mass+spectrometry+imaging+of+biological+tissue+sections+using+ice+as+a+matrix%5BTitle%5D)+AND+%22J+Am+Soc+Mass+Spectrom%22%5BJournal%5D)">IR-MALDESI mass spectrometry imaging of biological tissue sections using ice as a matrix.</a> J Am Soc Mass Spectrom. 25 (3), 319-328 (2014).</li>
<li>Barry, J. A., Groseclose, M. R., Robichaud, G., Castellino, S., Muddiman, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessing+drug+and+metabolite+detection+in+liver+tissue+by+uv-maldi+and+ir-maldesi+mass+spectrometry+imaging+coupled+to+ft-icr+ms%5BTitle%5D)+AND+%22Int+J+Mass+Spectrom%22%5BJournal%5D)">Assessing drug and metabolite detection in liver tissue by uv-maldi and ir-maldesi mass spectrometry imaging coupled to ft-icr ms.</a> Int J Mass Spectrom. 377, 448-155 (2015).</li>
<li>Pace, C. L., Muddiman, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Direct+analysis+of+native+N-linked+glycans+by+IR-MALDESI%5BTitle%5D)+AND+%22J+Am+Soc+Mass+Spectrom%22%5BJournal%5D)">Direct analysis of native N-linked glycans by IR-MALDESI.</a> J Am Soc Mass Spectrom. 31 (8), 1759-1762 (2020).</li>
<li>Palomino, T. V., Muddiman, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Predicting+sialic+acid+content+of+N-linked+glycans+using+the+isotopic+pattern+of+chlorine%5BTitle%5D)+AND+%22J+Am+Soc+Mass+Spectrom%22%5BJournal%5D)">Predicting sialic acid content of N-linked glycans using the isotopic pattern of chlorine.</a> J Am Soc Mass Spectrom. 34 (7), 1392-1399 (2023).</li>
<li>Mariethoz, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Glycomics%40expasy%3A+Bridging+the+gap%5BTitle%5D)+AND+%22Mol+Cell+Proteomics%22%5BJournal%5D)">Glycomics@expasy: Bridging the gap.</a> Mol Cell Proteomics. 17 (11), 2164-2176 (2018).</li>
<li>Varki, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Symbol+nomenclature+for+graphical+representations+of+glycans%5BTitle%5D)+AND+%22Glycobiology%22%5BJournal%5D)">Symbol nomenclature for graphical representations of glycans.</a> Glycobiology. 25 (12), 1323-1324 (2015).</li>
<li>Yu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+the+glycopattern+alterations+of+mitochondrial+proteins+in+cerebral+cortex+between+rat+alzheimer%27s+disease+and+the+cerebral+ischemia+model%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Comparison of the glycopattern alterations of mitochondrial proteins in cerebral cortex between rat alzheimer's disease and the cerebral ischemia model.</a> Sci Rep. 7 (1), 39948(2017).</li>
<li>Dixit, B., Vanhoozer, S., Anti, N. A., O'Connor, M. S., Boominathan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+enrichment+of+mitochondria+from+mammalian+cell+cultures+using+digitonin%5BTitle%5D)+AND+%22MethodsX%22%5BJournal%5D)">Rapid enrichment of mitochondria from mammalian cell cultures using digitonin.</a> MethodsX. 8, 101197(2021).</li>
<li>Kalathil, A. A., et al. New pathway for cisplatin prodrug to utilize metabolic substrate to overcome cancer intrinsic resistance. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aKalathil%2C+AA+%22central+science%22">central science</a>. 9, 1297-1312 (2023).</li>
<li>Hecht, E. S., McCord, J. P., Muddiman, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Definitive+screening+design+optimization+of+mass+spectrometry+parameters+for+sensitive+comparison+of+filter+and+solid+phase+extraction+purified%2C+inlight+plasma+N-glycans%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">Definitive screening design optimization of mass spectrometry parameters for sensitive comparison of filter and solid phase extraction purified, inlight plasma N-glycans.</a> Anal Chem. 87 (14), 7305-7312 (2015).</li>
<li>Hecht, E. S., McCord, J. P., Muddiman, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+quantitative+glycomics+and+proteomics+combined+purification+strategy%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">A quantitative glycomics and proteomics combined purification strategy.</a> J Vis Exp. (109), e53735(2016).</li>
<li>Fischler, D. A., Orlando, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((N-linked+glycan+release+efficiency%3A+A+quantitative+comparison+between+NaOCl+and+PNGase+F+release+protocols%5BTitle%5D)+AND+%22J+Biomol+Tech%22%5BJournal%5D)">N-linked glycan release efficiency: A quantitative comparison between NaOCl and PNGase F release protocols.</a> J Biomol Tech. 30 (4), 58-63 (2019).</li>
<li>Tu, A., Muddiman, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Internal+energy+deposition+in+infrared+matrix-assisted+laser+desorption+electrospray+ionization+with+and+without+the+use+of+ice+as+a+matrix%5BTitle%5D)+AND+%22J+Am+Soc+Mass+Spectrom%22%5BJournal%5D)">Internal energy deposition in infrared matrix-assisted laser desorption electrospray ionization with and without the use of ice as a matrix.</a> J Am Soc Mass Spectrom. 30 (11), 2380-2391 (2019).</li>
<li>Barry, J. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mapping+antiretroviral+drugs+in+tissue+by+IR-MALDESI+MSI+coupled+to+the+Q+exactive+and+comparison+with+LC-MS%2FMS+SRM+assay%5BTitle%5D)+AND+%22J+Am+Soc+Mass+Spectrom%22%5BJournal%5D)">Mapping antiretroviral drugs in tissue by IR-MALDESI MSI coupled to the Q exactive and comparison with LC-MS/MS SRM assay.</a> J Am Soc Mass Spectrom. 25 (12), 2038-2047 (2014).</li>
<li>Samal, J., Palomino, T. V., Chen, J., Muddiman, D. C., Segura, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Enhanced+detection+of+charged+N-glycans+in+the+brain+by+infrared+matrix-assisted+laser+desorption+electrospray+ionization+mass+spectrometric+imaging%5BTitle%5D)+AND+%22Anal+Chem%22%5BJournal%5D)">Enhanced detection of charged N-glycans in the brain by infrared matrix-assisted laser desorption electrospray ionization mass spectrometric imaging.</a> Anal Chem. 95 (29), 10913-10920 (2023).</li>
</ol>

<table><tbody><tr><td>Equipment</td><td></td><td></td><td></td></tr><tr><td>Amersham 600 imager</td><td>Cytvia&nbsp;</td><td>29194217</td><td>Gel and membrane imager</td></tr><tr><td>Countess 3 automated cell counter</td><td>Fisher Scientific</td><td>X003SZ1LY9</td><td></td></tr><tr><td>Dry Bath Stdrd 4 blck 100-120V</td><td>Thermofisher scientific&nbsp;</td><td>88870003</td><td></td></tr><tr><td>i-Blot2 Gel Transfer Device</td><td>Invitrogen</td><td>IB21001</td><td>Western blot transfer system</td></tr><tr><td>Inverted microscope</td><td>Cell Treat</td><td>04355223EA</td><td></td></tr><tr><td>Microplate reader</td><td></td><td>82050-760</td><td></td></tr><tr><td>Mini gel tank</td><td>Invitrogen</td><td>A25977</td><td></td></tr><tr><td>Open Air Rocker</td><td>Fisher Brand</td><td>88861025</td><td></td></tr><tr><td>Pipet boy&nbsp;</td><td>BioTek</td><td>229310</td><td></td></tr><tr><td>Vortex mixer</td><td>Integra- VWR</td><td></td><td></td></tr><tr><td>Mitochondria isolation reagents&nbsp;</td><td></td><td></td><td></td></tr><tr><td>Mitochondrial Isolation kit</td><td>Thermofisher scientific&nbsp;</td><td>89874</td><td></td></tr><tr><td>Phosphotase Inhibitor</td><td>Thermofisher scientific&nbsp;</td><td>1861274</td><td></td></tr><tr><td>Protease Inhibitor</td><td>Thermofisher scientific&nbsp;</td><td>1861281</td><td></td></tr><tr><td>N-glycan isolation and IR-MALDESI reagents</td><td></td><td></td><td></td></tr><tr><td>Acetic acid&nbsp;</td><td>Fisher Scientific</td><td>A11350</td><td>50% in ESI solvent</td></tr><tr><td>Acetonitrile&nbsp;</td><td>Sigma Aldrich</td><td>34851-4L</td><td>1 mM in ESI solvent</td></tr><tr><td>Ammonium bicarbonate&nbsp;</td><td>Fisher Scientific</td><td>A643500</td><td>100 mM</td></tr><tr><td>Calibration Solution</td><td>Thermofisher Scientific</td><td>A39239</td><td>Pierce FlexMix</td></tr><tr><td>Dithiothreitol&nbsp;</td><td>Sigma Aldrich</td><td>AC426380100</td><td>1 M</td></tr><tr><td>Iodoacetamide</td><td>Sigma Aldrich</td><td>A322-10VL</td><td></td></tr><tr><td>LC/MS grade water</td><td>Thermofisher Scientific</td><td>047146.M6</td><td></td></tr><tr><td>PNGase F</td><td>Bulldog Bio</td><td>NZPP010</td><td>75000 U/mL, enzyme for N-glycan release&nbsp;</td></tr><tr><td>N-glycan isolation and IR-MALDESI consumables</td><td></td><td></td><td></td></tr><tr><td>Amicon centrifugal filters</td><td>Fisher Scientific&nbsp;</td><td>UFC501024</td><td>10 kDa MWCO</td></tr><tr><td>Mass spectrometer</td><td>Orbitrap Exploris 240</td><td></td><td></td></tr><tr><td>Mid-IR Laser</td><td>JGM Associates, Burlington, MA, USA</td><td></td><td></td></tr><tr><td>Teflon microwell slide</td><td>Prosolia, Indianapolis, IN, USA</td><td></td><td></td></tr><tr><td>N-glycan analysis softwares</td><td></td><td></td><td></td></tr><tr><td>GlycoMod&nbsp;</td><td>Expasy&nbsp;</td><td></td><td>https://web.expasy.org/glycomod/</td></tr><tr><td>GlyConnect</td><td>Expasy&nbsp;</td><td></td><td>https://glyconnect.expasy.org/</td></tr><tr><td>Protein isolation and western blot consumables&nbsp;</td><td></td><td></td><td></td></tr><tr><td>Basix gel loading tips ( 10 &micro;L)&nbsp;</td><td>Basix</td><td>13-611-102</td><td></td></tr><tr><td>Basix gel loading tips ( 200 &micro;L)&nbsp;</td><td>Basix</td><td>13-611-116</td><td></td></tr><tr><td>Cell scrapper</td><td>VWR labs</td><td>14-388-100</td><td></td></tr><tr><td>i-Blot NC regular stacks</td><td>Invitrogen</td><td>IB23001</td><td></td></tr><tr><td>i-Blot2 PVDF Regular Stacks</td><td>Invitrogen</td><td>IB24001</td><td></td></tr><tr><td>10&nbsp; &micro;L micropipette</td><td>Fisher Scientific</td><td>FBE00010</td><td></td></tr><tr><td>20 &micro;L micropipette</td><td>Invitrogen</td><td>FBE00020</td><td></td></tr><tr><td>200&nbsp; &micro;L micropipette&nbsp;</td><td>Fisher Brand</td><td>FBE00200</td><td></td></tr><tr><td>1000 &micro;L micropipette</td><td>Fisher brand</td><td>FBE01000</td><td></td></tr><tr><td>10&nbsp; &micro;L pipet tips&nbsp;</td><td>VWR labs</td><td>76322-528</td><td></td></tr><tr><td>20&nbsp; &micro;L pipet tips</td><td>VWR labs</td><td>76322-134</td><td></td></tr><tr><td>200&nbsp; &micro;L pipet tips&nbsp;</td><td>VWR labs</td><td>76322-150</td><td></td></tr><tr><td>1000&nbsp; &micro;L pipet tips</td><td>VWR labs</td><td>76322-154</td><td></td></tr><tr><td>Well plate&nbsp;</td><td>Fisher brand</td><td>14-388-100</td><td></td></tr><tr><td>Protein isolation and western blot reagents&nbsp;</td><td></td><td></td><td></td></tr><tr><td>Actin antibody ( Host : Rabbit )</td><td>Cell Signaling Technologies</td><td>8457T</td><td></td></tr><tr><td>Anti-Rabbit IgG HRP Linked&nbsp;</td><td>Cell Signaling Technologies</td><td>7074S</td><td></td></tr><tr><td>Bolt 4-12% Bis-Tris Plus</td><td>Invitrogen</td><td>NW04120BOX</td><td></td></tr><tr><td>Bovine Serum Albumin</td><td>Fisher bioreagents</td><td>BP9700-100</td><td></td></tr><tr><td>COXIV antibody ( Host : Rabbit)</td><td>Cell Signaling Technologies</td><td>4844S</td><td></td></tr><tr><td>GAPDH antibody ( Host : Rabbit)</td><td>Cell Signaling Technologies</td><td>2118S</td><td></td></tr><tr><td>MicroBCA protein assay Kit</td><td>Thermofisher scientific&nbsp;</td><td>23235</td><td></td></tr><tr><td>Nupage MOPS SDS Runing Buffer [20x]</td><td>Thermofisher scientific&nbsp;</td><td>NP0001</td><td></td></tr><tr><td>PAGE Ruler prestained protein ladder</td><td>Thermofisher scientific&nbsp;</td><td>815-968-0747</td><td>Dilution= Use 7&nbsp; &micro;L to load onto first well</td></tr><tr><td>Phosphate buffered saline&nbsp;</td><td>Aniara Diagnostics</td><td>A12-9423-5</td><td>Prepare 1x PBS from 10x powder&nbsp;</td></tr><tr><td>Pierce ECL Western Blotting Substrate</td><td>Thermofisher scientific&nbsp;</td><td>32106</td><td>Chemiluminescent substrate kit</td></tr><tr><td>RIPA Buffer</td><td>Thermofisher scientific&nbsp;</td><td>89901</td><td></td></tr><tr><td>Sample Buffer</td><td>Novex</td><td>B0007</td><td>The bolt LDS sample buffer is prepared in 3:1 ratio of sample to sample buffer</td></tr><tr><td>Tween-20</td><td>MP Biomedicals</td><td>TWEEN201</td><td></td></tr><tr><td>Tissue culture consumables&nbsp;</td><td></td><td></td><td></td></tr><tr><td>Countess Slides</td><td>Avantor</td><td>229411</td><td></td></tr><tr><td>Eppendorf tubes</td><td>Cell Treat</td><td>414004-265612-5884</td><td></td></tr><tr><td>

mitochondriaal preparaat uit microglia voor glycaananalyse

Mitochondrial Preparation from Microglia for Glycan Analysis

Mitochondriaal preparaat uit microglia voor glycaananalyse

Understanding the glycosylation patterns of mitochondrial proteins in microglia is critical for determining their role in neurodegenerative diseases. ...

mitochondrial-preparation-from-microglia-for-glycan-analysis

Research

JoVE Journal

Bioengineering

Bio-engineering

Het isoleren van primaire microglia van de cellulaire heterogeniteit van de hersenen is essentieel om hun rol te onderzoeken in zowel fysiologische en pathologische omstandigheden. Dit protocol beschrijft een mechanische isolatie en gemengde celkweek techniek die hoge opbrengst en een hoge zuiverheid, levensvatbare primaire microgliale cellen...

Primaire Microglia Isolatie van Mixed gliale celculturen van neonatale rat hersenweefsel

Microglia zijn gevestigd macrofagen dat de eerste lijn van verdediging en het immuunsysteem de bewaking van het centrale zenuwstelsel te bieden. MicroRNA zijn moleculen die een belangrijke rol spelen in tal van fysiologische processen, zoals activatie en differentiatie van macrofagen. In dit artikel beschrijven we de methode voor het meten van microRNA's in de microglia.

Detectie van microRNA&#39;s in Microglia door Real-time PCR in Normal CNS en tijdens ontstekingsprocessen

The video demonstrates a technique for isolating microglia from the cortex of an adult mouse brain. First, the cortex undergoes segmentation and homogenization to remove neurons and other glial cells, preserving microglia. Following that, the cell suspension is exposed to anti-myelin magnetic microbeads and a magnetic field, effectively separating microglia and myeloid cells from myelin debris and cells containing myelin.

Isolation of Microglia from the Cortex of an Adult Mouse Brain

Deze procedure laat zien hoe u te isoleren van de volwassen hersenen van muizen de mitochondriën-geassocieerde ER membranen of Mams en de glycosfingolipiden verrijkte microdomain fracties uit Mams en mitochondriale preparaten.

Mitochondriën-geassocieerde ER Membranen (eigen MTM) en glycosfingolipiden Verrijkt microdomeinen (GEM): Isolatie van Mouse Brain

Schetsen we methoden voor de efficiënte en snelle isolatie / cultuur van levensvatbare microglia uit de neonatale cerebrale cortex en volwassen ruggenmerg. De dissectie en beplating van corticale microglia kan worden bereikt binnen 90 minuten, met de daaropvolgende microglia oogst plaatsvindt ~ 10 dagen na de eerste dissectie.

Kweken Microglia van de neonatale en volwassen centrale zenuwstelsel

Een sleutel tot succesvolle onderzoek van microglia biologie is het behoud van microglia immunofunction ex vivo tijdens isolatie van CZS-weefsel. Isoleren microglia via roterende schudden resulteert in zeer zuivere en immunofunctional celculturen zoals beoordeeld door fluorescerende beeldvorming, immunocytochemie, en ELISA na microglia-activatie met de pro-inflammatoire stimuli lipopolysaccharide (LPS) en Pam 3 CSK 4 (Pam).

Isolatie van corticale Microglia met Conserven immunofenotype en functionaliteit van Muriene Pasgeborenen

Hier, wordt een protocol (MitoCeption) voorgelegd aan mitochondriën geïsoleerd uit menselijke mesenchymale stamcellen (MSC) over te dragen, glioblastoma stamcellen (GSC), met als doel het bestuderen van hun biologische effecten op het metabolisme en de functies SGR. Een soortgelijk protocol kan worden aangepast aan mitochondriën dragen tussen andere celtypen.

MitoCeption: overbrengen geïsoleerde menselijke MSC Mitochondriën aan glioblastoma Stamcellen

Hier is een protocol voor het onderzoek van de interacties tussen inheemse vorm, prefibrillar, en volwassen amyloïde fibrillen van verschillende peptiden en eiwitten met mitochondriën geïsoleerd uit verschillende weefsels en verschillende gebieden van de hersenen.

Interacties met en membraan Permeabilization van de mitochondriën van de hersenen door amyloid fibrils

We bieden een protocol voor isolatie van Microglia uit verschillende ontleed gebieden van een volwassen muis hersen halfrond, gevolgd door semi-geautomatiseerde bibliotheek voorbereiding voor diepe eencellige RNA-sequencing van volledige lengte transcriptomes. Deze methode zal helpen om functionele heterogeniteit van Microglia in gezondheid en ziekte te verhelmaken.

Isolatie van regiospecifieke Microglia van één volwassen muis hersen halfrond voor diepe eencellige RNA-sequencing

Deze studie presenteert een methode om de morfologie van mitochondriën te analyseren op basis van immunokleuring en beeldanalyse in hersenweefsel van muizen in situ. Het beschrijft ook hoe dit het mogelijk maakt om veranderingen in de mitochondriale morfologie te detecteren die worden veroorzaakt door eiwitaggregatie in modellen voor de ziekte van Parkinson.

Histologisch onderzoek van mitochondriale morfologie in een model voor de ziekte van Parkinson

Dit protocol beschrijft een kosteneffectieve manier om een glycolyse-stresstest uit te voeren om metabole herprogrammering in primaire muismicroglia te kwantificeren. Na isolatie en ontstekingsstimulatie wordt de extracellulaire verzuringssnelheid (ECAR) gemeten met vers bereide, pH-gecorrigeerde reagentia, gevolgd door eiwitnormalisatie om een nauwkeurige en reproduceerbare beoordeling van glycolytische verschuivingen mogelijk te maken.

Onderzoek naar glycolyse in primaire microglia met behulp van extracellulaire fluxassay

Leer hoe je mitochondria isoleert uit BV-2 microglia voor glycaananalyse met infrarood-MALDESI-massaspectrometrie. Deze methode helpt onderzoekers de rol van glycanen in neuro-immunologische interacties te begrijpen.

Mitochondriaal preparaat uit microglia voor glycaananalyse

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