Name: Subnanometer-resolutie structurele bepaling van hemagglutinine uit cryo-elektronentomografie van griepvirussen (Video) | JoVE Journal
Uploaded: 2025-11-07T12:50:00.000+00:00
Duration: 8 min 19 s
Description: Leer hoe je cryo-elektronentomografie gebruikt om hemagglutinine van griepvirus te bestuderen op subnanometer-resolutie. Deze methode onthult verborgen interacties tijdens receptorbinding.

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<table><tbody><tr><td>AMD Ryzen Threadripper PRO 5965WX</td><td>AMD</td><td>https://www.amd.com/en/support/downloads/drivers.html/processors/ryzen-threadripper-pro/ryzen-threadripper-pro-5000wx-series/amd-ryzen-threadripper-pro-5965wx.html</td><td></td></tr><tr><td>AreTomo 1.3.4</td><td>UC San Francisco</td><td>https://drive.google.com/drive/folders/1Z7pKVEdgMoNaUmd_cOFhlt-QCcfcwF3_</td><td></td></tr><tr><td>EMAN2 2.99.52</td><td>Baylor College of Medicine</td><td>https://blake.bcm.edu/emanwiki/EMAN2</td><td></td></tr><tr><td>IMOD 4.12.27</td><td>University of Colorado at Boulder</td><td>https://bio3d.colorado.edu/imod/</td><td></td></tr><tr><td>Influenza analysis scripts</td><td>UMass Chan Medical School</td><td>https://github.com/jqyhuang/influenza-analysis</td><td></td></tr><tr><td>IsoNet 0.3</td><td>UCLA</td><td>https://github.com/IsoNet-cryoET/IsoNet</td><td></td></tr><tr><td>M 2.0.0</td><td>Genentech</td><td>https://warpem.github.io/warp/home/m/</td><td></td></tr><tr><td>NVIDIA A4000</td><td>NVIDIA&nbsp;</td><td>https://www.nvidia.com/en-us/products/workstations/rtx-a4000/</td><td></td></tr><tr><td>Open3D&nbsp;</td><td>Intel Labs</td><td>https://www.open3d.org/</td><td></td></tr><tr><td>PHENIX 1.21-5207</td><td>Lawrence Berkeley National Laboratory</td><td>phenix-online.org</td><td></td></tr><tr><td>RELION 4.0</td><td>MRC Laboratory of Molecular Biology</td><td>https://relion.readthedocs.io/en/release-4.0/</td><td></td></tr><tr><td>Ubuntu 20.04</td><td>Ubuntu</td><td>https://releases.ubuntu.com/focal/</td><td></td></tr><tr><td>UCSF ChimeraX 1.6.1</td><td>UC San Francisco</td><td>https://www.cgl.ucsf.edu/chimerax/</td><td></td></tr><tr><td>Warp 2.0.0</td><td>Genentech</td><td>http://warpem.github.io/warp/</td><td></td></tr></tbody></table>

subnanometer-resolutie structurele bepaling van hemagglutinine uit cryo-elektronentomografie van griepvirussen

Subnanometer-Resolution Structural Determination of Hemagglutinin from Cryo-Electron Tomography of Influenza Viruses

Subnanometer-resolutie structurele bepaling van hemagglutinine uit cryo-elektronentomografie van griepvirussen

Cryo-electron tomography is a powerful tool to visualize heterogeneous samples, with one major application being structural characterization of ...

subnanometer-resolution-structural-determination-hemagglutinin-from

Research

JoVE Journal

Biochemistry

Biochemie

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We beschrijven een correlatieve microscopie methode die high-speed 3D levende cellen fluorescerend licht microscopie en hoge-resolutie cryo-electron tomografie combineert. We demonstreren het vermogen van de correlatieve wijze dynamische beeldvorming, kleine HIV-1 deeltjes interactie met gastheer HeLa cellen.

Correlatieve microscopie voor 3D Structural Analysis van Dynamic Interactions

We presenteren een protocol over hoe om te verzamelen en te verwerken elektronen cryo-tomogrammen van hele mitochondriën. De techniek biedt gedetailleerd inzicht in de structuur, functie en organisatie van grote membraan eiwitcomplexen in de moedertaal van biologische membranen.

Visualisatie van ATP synthase Dimeren in Mitochondriën door Electron Cryo-tomografie

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Een gemiddelde van virale envelop glycoproteïne Spikes uit Electron Cryotomography Reconstructies met Jsubtomo

In dit artikel wordt een protocol voor het verwerken van cryo-EM-beelden gepresenteerd met behulp van de software suite SPHIRE. Het onderhavige protocol kan toegepast worden op bijna alle enkel-deeltjes-EM-projecten die zich richten op bijna atomaire resolutie.

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Bereiding en Cryo-FIB microbewerking van Saccharomyces cerevisiae voor Cryo-Elektronen Tomografie

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Geavanceerde beeldvorming met hoge resolutie van virusassemblages in vloeistof en ijs

Het huidige protocol beschrijft cryo-elektronentomografie op afstand met hoge resolutie data-acquisitie met behulp van Tomo5 en daaropvolgende gegevensverwerking en subtomogrammiddeling met behulp van emClarity. Apoferritine wordt als voorbeeld gebruikt om gedetailleerde stapsgewijze processen te illustreren om een cryo-ET-structuur met een resolutie van 2,86 Å te bereiken.

Cryo-elektronentomografie Gegevensverzameling op afstand en subtomogrammiddeling

Dit videoprotocol illustreert hoe scanning-transmissie-elektronenmicroscopietomografie van virologische monsters moet worden uitgevoerd. Voor optimale resultaten worden monsters bereid door vries onder hoge druk en daaropvolgende vriessubstitutie.

Scanning transmissie-elektronenmicroscopietomografie in de virologie: 3D-beeldvorming van onder hoge druk ingevroren, vriesgesubstitueerde monsters

Leer hoe je cryo-elektronentomografie gebruikt om hemagglutinine van griepvirus te bestuderen op subnanometer-resolutie. Deze methode onthult verborgen interacties tijdens receptorbinding.

Subnanometer-resolutie structurele bepaling van hemagglutinine uit cryo-elektronentomografie van griepvirussen

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