Name: Witte en bruine vettransplantaten: een benadering om reproductieve, metabole en niertekorten te corrigeren bij zwarte en bruine brachyury (BTBR) zwaarlijvige muizen (Video) | JoVE Journal
Uploaded: 2025-09-09T13:45:00.000+00:00
Duration: 6 min 16 s
Description: Leer een gestandaardiseerd protocol voor vettransplantatie bij BTBR zwaarlijvige muizen. Deze methode verbetert metabole gezondheid, nierfunctie en vruchtbaarheid door wit en bruin vetweefsel te transplanteren.

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5 minutes

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<li>GBD 2021 Adult BMI Collaborators. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adult+BMI+Collaborators.+Global%2C+regional%2C+and+national+prevalence+of+adult+overweight+and+obesity%2C+1990-2021%2C+with+forecasts+to+2050%3A+a+forecasting+study+for+the+Global+Burden+of+Disease+Study+2021%5BTitle%5D)+AND+%22Lancet%22%5BJournal%5D)">Adult BMI Collaborators. Global, regional, and national prevalence of adult overweight and obesity, 1990-2021, with forecasts to 2050: a forecasting study for the Global Burden of Disease Study 2021.</a> Lancet. 405 (10481), 813-838 (2025).</li>
<li>Liu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Brown+adipose+tissue+transplantation+reverses+obesity+in+Ob%2FOb+mice%5BTitle%5D)+AND+%22Endocrinology%22%5BJournal%5D)">Brown adipose tissue transplantation reverses obesity in Ob/Ob mice.</a> Endocrinology. 156 (7), 2461-2469 (2015).</li>
<li>Ghesmati, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+update+on+the+secretory+functions+of+brown%2C+white%2C+and+beige+adipose+tissue%3A+Towards+therapeutic+applications%5BTitle%5D)+AND+%22Rev+Endocr+Metab+Disord%22%5BJournal%5D)">An update on the secretory functions of brown, white, and beige adipose tissue: Towards therapeutic applications.</a> Rev Endocr Metab Disord. 25 (2), 279-308 (2024).</li>
<li>Dewal, R. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Transplantation+of+committed+pre-adipocytes+from+brown+adipose+tissue+improves+whole-body+glucose+homeostasis%5BTitle%5D)+AND+%22iScience%22%5BJournal%5D)">Transplantation of committed pre-adipocytes from brown adipose tissue improves whole-body glucose homeostasis.</a> iScience. 27 (2), 108927(2024).</li>
<li>Klebanov, S., Astle, C. M., DeSimone, O., Ablamunits, V., Harrison, D. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adipose+tissue+transplantation+protects+ob%2Fob+mice+from+obesity%2C+normalizes+insulin+sensitivity+and+restores+fertility%5BTitle%5D)+AND+%22J+Endocrinol%22%5BJournal%5D)">Adipose tissue transplantation protects ob/ob mice from obesity, normalizes insulin sensitivity and restores fertility.</a> J Endocrinol. 186 (1), 203-211 (2005).</li>
<li>Childs, G. V., Odle, A. K., MacNicol, M. C., MacNicol, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+importance+of+leptin+to+reproduction%5BTitle%5D)+AND+%22Endocrinology%22%5BJournal%5D)">The importance of leptin to reproduction.</a> Endocrinology. 162 (2), 1-18 (2021).</li>
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<li>Stanford, K. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Brown+adipose+tissue+regulates+glucose+homeostasis+and+insulin+sensitivity%5BTitle%5D)+AND+%22J+Clin+Invest%22%5BJournal%5D)">Brown adipose tissue regulates glucose homeostasis and insulin sensitivity.</a> J Clin Invest. 123 (1), 215-223 (2013).</li>
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<li>Valgas da Silva, P. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Brown+adipose+tissue+prevents+glucose+intolerance+and+cardiac+remodeling+in+high-fat-fed+mice+after+a+mild+myocardial+infarction%5BTitle%5D)+AND+%22Int+J+Obes+%28Lond%29%22%5BJournal%5D)">Brown adipose tissue prevents glucose intolerance and cardiac remodeling in high-fat-fed mice after a mild myocardial infarction.</a> Int J Obes (Lond). 46 (2), 350-358 (2022).</li>
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<li>Clee, S. M., Nadler, S. T., Attie, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Genetic+and+genomic+studies+of+the+BTBR+ob%2Fob+mouse+model+of+type+2+diabetes%5BTitle%5D)+AND+%22Am+J+Ther%22%5BJournal%5D)">Genetic and genomic studies of the BTBR ob/ob mouse model of type 2 diabetes.</a> Am J Ther. 12 (6), 491-498 (2005).</li>
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<li>Liu, X., Zhang, Z., Song, Y., Xie, H., Dong, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+update+on+brown+adipose+tissue+and+obesity+intervention%3A+Function%2C+regulation+and+therapeutic+implications%5BTitle%5D)+AND+%22Front+Endocrinol+%28Lausanne%29%22%5BJournal%5D)">An update on brown adipose tissue and obesity intervention: Function, regulation and therapeutic implications.</a> Front Endocrinol (Lausanne). 13, 1065263(2022).</li>
<li>Mollah, M. L., Yang, H. S., Jeon, S., Kim, K., Cheon, Y. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Overaccumulation+of+fat+caused+rapid+reproductive+senescence+but+not+loss+of+ovarian+reserve+in+ob%2Fob+mice%5BTitle%5D)+AND+%22J+Endocr+Soc%22%5BJournal%5D)">Overaccumulation of fat caused rapid reproductive senescence but not loss of ovarian reserve in ob/ob mice.</a> J Endocr Soc. 5 (1), bvaa168(2021).</li>
<li>Serke, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Leptin-deficient+%28ob%2Fob%29+mouse+ovaries+show+fatty+degeneration%2C+enhanced+apoptosis+and+decreased+expression+of+steroidogenic+acute+regulatory+enzyme%5BTitle%5D)+AND+%22Int+J+Obes+%28Lond%29%22%5BJournal%5D)">Leptin-deficient (ob/ob) mouse ovaries show fatty degeneration, enhanced apoptosis and decreased expression of steroidogenic acute regulatory enzyme.</a> Int J Obes (Lond). 36 (8), 1047-1053 (2012).</li>
<li>Diamanti-Kandarakis, E., Dunaif, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Insulin+resistance+and+the+polycystic+ovary+syndrome+revisited%3A+an+update+on+mechanisms+and+implications%5BTitle%5D)+AND+%22Endocr+Rev%22%5BJournal%5D)">Insulin resistance and the polycystic ovary syndrome revisited: an update on mechanisms and implications.</a> Endocr Rev. 33 (6), 981-1030 (2012).</li>
<li>Chang, A. S., Dale, A. N., Moley, K. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Maternal+diabetes+adversely+affects+preovulatory+oocyte+maturation%2C+development%2C+and+granulosa+cell+apoptosis%5BTitle%5D)+AND+%22Endocrinology%22%5BJournal%5D)">Maternal diabetes adversely affects preovulatory oocyte maturation, development, and granulosa cell apoptosis.</a> Endocrinology. 146 (5), 2445-2453 (2005).</li>
<li>Almog, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Leptin+attenuates+follicular+apoptosis+and+accelerates+the+onset+of+puberty+in+immature+rats%5BTitle%5D)+AND+%22Mol+Cell+Endocrinol%22%5BJournal%5D)">Leptin attenuates follicular apoptosis and accelerates the onset of puberty in immature rats.</a> Mol Cell Endocrinol. 183 (1-2), 179-191 (2001).</li>
<li>Beyerstedt, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Combining+sodium-glucose+co-transporter-2+inhibitor+with+mesenchymal+stem+cells+and+brown+adipose+tissue+%28BAT%29+and+white+adipose+tissue+%28WAT%29+transplantation+to+mitigate+the+progression+of+diabetic+kidney+disease%3A+a+pre-clinical+approach%5BTitle%5D)+AND+%22Stem+Cell+Res+Ther%22%5BJournal%5D)">Combining sodium-glucose co-transporter-2 inhibitor with mesenchymal stem cells and brown adipose tissue (BAT) and white adipose tissue (WAT) transplantation to mitigate the progression of diabetic kidney disease: a pre-clinical approach.</a> Stem Cell Res Ther. 16 (254), 1-15 (2025).</li>
<li>Sávio-Silva, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Therapeutic+potential+of+mesenchymal+stem+cells+in+a+pre-clinical+model+of+diabetic+kidney+disease+and+obesity%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Therapeutic potential of mesenchymal stem cells in a pre-clinical model of diabetic kidney disease and obesity.</a> Int J Mol Sci. 22 (4), 1546(2021).</li>
<li>Takemura, S., Shimizu, T., Oka, M., Sekiya, S., Babazono, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Transplantation+of+adipose-derived+mesenchymal+stem+cell+sheets+directly+into+the+kidney+suppresses+the+progression+of+renal+injury+in+a+diabetic+nephropathy+rat+model%5BTitle%5D)+AND+%22J+Diabetes+Investig%22%5BJournal%5D)">Transplantation of adipose-derived mesenchymal stem cell sheets directly into the kidney suppresses the progression of renal injury in a diabetic nephropathy rat model.</a> J Diabetes Investig. 11 (3), 545-553 (2020).</li>
<li>Pichaiwong, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reversibility+of+structural+and+functional+damage+in+a+model+of+advanced+diabetic+nephropathy%5BTitle%5D)+AND+%22J+Am+Soc+Nephrol%22%5BJournal%5D)">Reversibility of structural and functional damage in a model of advanced diabetic nephropathy.</a> J Am Soc Nephrol. 24 (7), 1088-1102 (2013).</li>
<li>Almog, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Leptin+attenuates+follicular+apoptosis+and+accelerates+the+onset+of+puberty+in+immature+rats%5BTitle%5D)+AND+%22Mol+Cell+Endocrinol%22%5BJournal%5D)">Leptin attenuates follicular apoptosis and accelerates the onset of puberty in immature rats.</a> Mol Cell Endocrinol. 183 (1-2), 179-191 (2001).</li>
<li>O'Brien, P. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((BTBR+ob%2Fob+mice+as+a+novel+diabetic+neuropathy+model%3A+Neurological+characterization+and+gene+expression+analysis%5BTitle%5D)+AND+%22Neurobiol+Dis%22%5BJournal%5D)">BTBR ob/ob mice as a novel diabetic neuropathy model: Neurological characterization and gene expression analysis.</a> Neurobiol Dis. 73, 348-355 (2015).</li>
<li>Sávio-Silva, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mesenchymal+stem+cell+therapy+for+diabetic+kidney+disease%3A+A+review+of+the+studies+using+syngeneic%2C+autologous%2C+allogenic%2C+and+xenogenic+cells%5BTitle%5D)+AND+%22Stem+Cells+Int%22%5BJournal%5D)">Mesenchymal stem cell therapy for diabetic kidney disease: A review of the studies using syngeneic, autologous, allogenic, and xenogenic cells.</a> Stem Cells Int. 2020, 8833725(2020).</li>
<li>Morsy, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Leptin+gene+therapy+and+daily+protein+administration%3A+a+comparative+study+in+the+ob%2Fob+mouse%5BTitle%5D)+AND+%22Gene+Ther%22%5BJournal%5D)">Leptin gene therapy and daily protein administration: a comparative study in the ob/ob mouse.</a> Gene Ther. 5 (1), 8-18 (1998).</li>
<li>Wang, Y., Asakawa, A., Inui, A., Kosai, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Leptin+gene+therapy+in+the+fight+against+diabetes%5BTitle%5D)+AND+%22Expert+Opin+Biol+Ther%22%5BJournal%5D)">Leptin gene therapy in the fight against diabetes.</a> Expert Opin Biol Ther. 10 (10), 1405-1414 (2010).</li>
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</ol>

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witte en bruine vettransplantaten: een benadering om reproductieve, metabole en niertekorten te corrigeren bij zwarte en bruine brachyury (btbr) zwaarlijvige muizen

White and Brown Adipose Grafts: An Approach to Correct Reproductive, Metabolic, and Renal Deficits in Black and Tan Brachyury (BTBR) Obese Mice

Witte en bruine vettransplantaten: een benadering om reproductieve, metabole en niertekorten te corrigeren bij zwarte en bruine brachyury (BTBR) zwaarlijvige muizen

Transplantation of white adipose tissue (WAT) and brown adipose tissue (BAT) has emerged as a promising therapeutic strategy for reversing metabolic ...

white-brown-adipose-grafts-an-approach-to-correct-reproductive

Research

JoVE Journal

Biology

Biologie

In this video, we demonstrate the surgical procedure to isolate the subcutaneous white adipose tissue and interscapular brown adipose tissue from a newborn mouse.

White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup

Primaire witte preadipocyten geïsoleerd van wit vetweefsel in muizen kunnen worden onderscheiden in beige / brite cellen. Hier wordt gepresenteerd is een betrouwbare cellulaire modelsysteem om de moleculaire regulatie van "bruinen" van wit vet te bestuderen.

Isolatie en differentiatie van Stroma vasculaire cellen te Beige / Brite Cellen

Dit artikel beschrijft een transplantatiemethode om donorrattenborstepitheelcellen te transplanteren in het interscapulaire witte vetkussen van de ontvangende dieren. Deze methode kan worden gebruikt om gastheer- en/of donoreffecten op de ontwikkeling van borstepitheel te onderzoeken en elimineert de noodzaak van pre-clearing, waardoor het nut van deze techniek wordt uitgebreid.

Rat Mammary Epitheliale Celtransplantatie in de Interscapular White Fat Pad

Dit rapport beschrijft een protocol voor de gelijktijdige isolatie van primaire bruine en witte preadipocyten van pasgeboren muizen. Geïsoleerde cellen kunnen in cultuur worden gekweekt en worden geïnduceerd om zich te onderscheiden in volgroeide witte en bruine adipocyten. De methode maakt genetische, moleculaire en functionele karakterisering van primaire vetcellen in cultuur mogelijk.

Isolatie en differentiatie van primaire witte en bruine preadipocyten van pasgeboren muizen

Functionele beeldvorming en kwantificering van thermogene vetdepots bij muizen met behulp van een micro-PET / MR-beeldvormingsgebaseerde benadering.

Visualisatie en kwantificering van bruine en beige vetweefsels bij muizen met behulp van [18F] FDG Micro-PET / MR Imaging

Preadipocyten worden geïsoleerd uit de stromale vasculaire fractie van interscapulier bruin vetweefsel van pasgeboren muizen en gedifferentieerd tot cellen die lipidedruppeltjes accumuleren, moleculaire markers tot expressie brengen en de mitochondriale morfologie van volwassen bruine vetcellen vertonen. Deze cellen worden verder geanalyseerd door middel van immunofluorescentie en transmissie-elektronenmicroscopie.

Differentiatie en beeldvorming van bruine vetcellen uit de stromale vasculaire fractie van interscapulier vetweefsel van pasgeboren muizen

Hier presenteren we een gedetailleerd protocol dat het gebruik van microvasculaire fragmenten geïsoleerd uit knaagdier- of menselijk vetweefsel schetst als een eenvoudige benadering om functioneel, gevasculariseerd beige vetweefsel te engineeren.

Driedimensionale kweek van gevasculariseerd thermogeen vetweefsel uit microvasculaire fragmenten

Dit protocol presenteert het gebruik van CRISPR SunTag-p65-HSF1 (SPH) in adipocyten (AdipoSPH) als een alternatieve strategie voor adeno-geassocieerd virus (AAV) voor het onderzoeken van beige vetbiologie. In vivo injectie van AAV-dragend sgRNA gericht op het endogene Prdm16-gen is voldoende om de ontwikkeling van beige vet te induceren en het thermogene genprogramma te verbeteren.

Onderzoek naar beige vetbiologie en metabolisme met behulp van het CRISPR SunTag-p65-HSF1-activeringssysteem

In dit protocol beschrijven we de technische procedures om bruin vetweefsel (BAT)-specifieke knock-out muizen te genereren met behulp van een gecombineerd Cre-LoxP, CRISPR-Cas9 en adeno-geassocieerd virus (AAV) single-guide RNA (sgRNA) systeem. De beschreven stappen omvatten het ontwerp van de sgRNA's, de bereiding van de AAV-sgRNA-deeltjes en de micro-injectie van AAV in de BBT-lobben.

Generatie van bruine vet-specifieke knock-out muizen met behulp van een gecombineerd Cre-LoxP, CRISPR-Cas9 en Adeno-geassocieerd virus single-guide RNA-systeem

Genetische Induceerbare Fate Mapping (GIFM) merken en tracks cellen met fijne ruimtelijke en temporele...

Een praktische benadering van genetische Induceerbare Fate Mapping: een visuele gids voor Mark en Track Cellen In Vivo</em

Leer een gestandaardiseerd protocol voor vettransplantatie bij BTBR zwaarlijvige muizen. Deze methode verbetert metabole gezondheid, nierfunctie en vruchtbaarheid door wit en bruin vetweefsel te transplanteren.

Witte en bruine vettransplantaten: een benadering om reproductieve, metabole en niertekorten te corrigeren bij zwarte en bruine brachyury (BTBR) zwaarlijvige muizen (Video) | JoVE Journal

Witte en bruine vettransplantaten: een benadering om reproductieve, metabole en niertekorten te corrigeren bij zwarte en bruine brachyury (BTBR) zwaarlijvige muizen

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