Name: Gecombineerde recombinasepolymeraseamplificatie CRISPR/Cas12a-test voor het detecteren van Fusarium oxysporum f. sp. cubense Tropical Race 4 (Video) | JoVE Journal
Uploaded: 2025-11-14T13:35:00.000+00:00
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Description: Leer een snelle CRISPR/Cas12a-detectiemethode voor Fusarium oxysporum f. sp. cubense Tropical Race 4 in bananen. Deze techniek combineert recombinasepolymeraseamplificatie met draagbare fluorescentiedetectie voor kosteneffectieve velddiagnostiek.

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<li>Zhang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+and+sensitive+detection+of+toxigenic+Fusarium+asiaticum+integrating+recombinase+polymerase+amplification%2C+CRISPR%2FCas12a%2C+and+lateral+flow+techniques%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Rapid and sensitive detection of toxigenic Fusarium asiaticum integrating recombinase polymerase amplification, CRISPR/Cas12a, and lateral flow techniques.</a> Int J Mol Sci. 24 (18), 14134(2023).</li>
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<table><tbody><tr><td>EnGen Lba Cas12a (Cfp1) Protein</td><td>New England Biolabs</td><td>M0653T</td><td></td></tr><tr><td>EnGen Lba Cas12a Diluent</td><td>New England Biolabs</td><td>B0653A</td><td></td></tr><tr><td>Eppendorf PCR Tubes</td><td>Merck</td><td>EP0030124332</td><td></td></tr><tr><td>Eppendorf Safe-Lock Micro Test Tubes</td><td>Merck</td><td>EP0030121708</td><td></td></tr><tr><td>Extraction Bags Universal</td><td>BIOREBA</td><td>430100</td><td></td></tr><tr><td>GeneRuler 100 bp DNA Ladder</td><td>Thermo Fischer Scientific</td><td>SM0241 / SM0242</td><td></td></tr><tr><td>Genie Centrifuge Mini-6KO</td><td>OptiGene</td><td>OP-FUGE</td><td></td></tr><tr><td>Genie III</td><td>OptiGene</td><td>https://www.optigene.co.uk/instruments/genie-iii/&nbsp;</td><td></td></tr><tr><td>Genie Strip Holder</td><td>OptiGene</td><td>GBLOCK-01 / GBLOCK-02 / GBLOCK-03</td><td></td></tr><tr><td>Genie Strips</td><td>OptiGene</td><td>OP-0008-50 / OP-0008-500</td><td></td></tr><tr><td>HiScribe T7 High Yield RNA Synthesis Kit</td><td>New England Biolabs</td><td>E2040S</td><td></td></tr><tr><td>NanoDrop ND-1000 UV/Vis Spectrophotometer</td><td>Thermo Fischer Scientific</td><td>https://caeonline.com/buy/spectrometers/nanodrop-nd-1000/293669253</td><td></td></tr><tr><td>NEBuffer 3</td><td>New England Biolabs</td><td>B7003S</td><td></td></tr><tr><td>NEBuffer r2.1</td><td>New England Biolabs</td><td>B6002S</td><td></td></tr><tr><td>Polyvinylpyrrolidone (PVP) powder</td><td>Sigma-Aldrich</td><td>9003-39-8</td><td></td></tr><tr><td>RNA Clean and Concentrator-5 Kit</td><td>Zymo Research</td><td>R1013</td><td></td></tr><tr><td>RNA Loading Dye</td><td>Thermo Fischer Scientific</td><td>R0641</td><td></td></tr><tr><td>SmartBlue Blue Light Transilluminator</td><td>Accuris Instruments</td><td>E4000-E</td><td></td></tr><tr><td>Sodium hydroxide (NaOH) pellets</td><td>Merck, EMPARTA</td><td>K277</td><td></td></tr><tr><td>Tris (hydroxylmethyl) aminomethane</td><td>Merck</td><td>77-86-1</td><td></td></tr><tr><td>TwistAmp Liquid Basic Kit</td><td>TwistDx</td><td>TALQBAS01</td><td></td></tr></tbody></table>

gecombineerde recombinasepolymeraseamplificatie crispr/cas12a-test voor het detecteren van fusarium oxysporum f. sp. cubense tropical race 4

Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4

Gecombineerde recombinasepolymeraseamplificatie CRISPR/Cas12a-test voor het detecteren van Fusarium oxysporum f. sp. cubense Tropical Race 4

Regular and accurate surveillance stands central to the efficient management of plant diseases. It can indicate which course of action is most ...

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Leer een snelle CRISPR/Cas12a-detectiemethode voor Fusarium oxysporum f. sp. cubense Tropical Race 4 in bananen. Deze techniek combineert recombinasepolymeraseamplificatie met draagbare fluorescentiedetectie voor kosteneffectieve velddiagnostiek.

Gecombineerde recombinasepolymeraseamplificatie CRISPR/Cas12a-test voor het detecteren van Fusarium oxysporum f. sp. cubense Tropical Race 4 (Video) | JoVE Journal

Gecombineerde recombinasepolymeraseamplificatie CRISPR/Cas12a-test voor het detecteren van Fusarium oxysporum f. sp. cubense Tropical Race 4

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