Name: Vergelijkende studie van het gehalte aan polysachariden en de antioxiderende werking van Hippophae rhamnoides subsp. sinensis en Hippophae gyantsensis (Video) | JoVE Journal
Uploaded: 2025-08-15T14:30:00.000+00:00
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Description: Ontdek de vergelijking van polysachariden en antioxiderende werking bij Hippophae rhamnoides subsp. sinensis en Hippophae gyantsensis. Deze methode helpt onderzoekers bij kwaliteitsbeoordeling voor farmaceutische toepassingen.

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</ol>

<table><tbody><tr><td>Analytical balance (met een precisie van 0,0001 g)</td><td>Sartorius Scientific Instruments (Beijing) Co., Ltd.</td><td>BSA224S-CW</td><td></td></tr><tr><td>Analytical balance (met een precisie van 0,00001 g)</td><td>Sartorius Scientific Instruments (Beijing) Co., Ltd.</td><td>CPA225D</td><td></td></tr><tr><td>Benchtop laagsnelheidscentrifuge</td><td>Shanghai Zigui Instrument Co., Ltd.</td><td>TD4K-Z</td><td></td></tr><tr><td>DPPH</td><td>Shanghai Yuanye Bio-Technology Co., Ltd.</td><td>CAS 1898-66-4</td><td></td></tr><tr><td>Ethanol</td><td>Chengdu Kelong Chemical Products Co., Ltd.</td><td>CAS 64-17-5</td><td></td></tr><tr><td>EP tube</td><td>Hunan BKMAK Holding Co., Ltd.</td><td>BS-20-M</td><td>2 mL</td></tr><tr><td>Vriesdroger</td><td>Labconco Corporation</td><td>LABCONCO</td><td></td></tr><tr><td>Glucose anhydrous</td><td>Chengdu Kelong Chemical Products Co., Ltd.</td><td>CAS 50-99-7</td><td>Referentiestandaard</td></tr><tr><td>GraphPad&nbsp; Prim 9.5.0&nbsp;</td><td>GraphPad Software</td><td>N/A</td><td>Bepaal de IC50-waarde en de P-waarde.</td></tr><tr><td>Heteluchtcirculatiedroger</td><td>Shanghai Yiheng Technology Instrument Co., Ltd.</td><td>NB-DGG-9070A</td><td></td></tr><tr><td>L-Ascorbinezuur</td><td>Beijing Labgic Technology Co., Ltd.</td><td>CAS 50-81-7</td><td>Positieve controledrug</td></tr><tr><td>Microplaatlezer</td><td>Shanghai Shanpu Biotechnology Co., Ltd.</td><td>Read Max 1500</td><td></td></tr><tr><td>96-well plate</td><td>Beijing Labgic Technology Co., Ltd.</td><td>11512</td><td></td></tr><tr><td>OriginPro 2025</td><td>OriginLab</td><td>N/A</td><td>Maak een clearantiepercentage-lijngrafiek</td></tr><tr><td>PIPETTIPJES</td><td>Beijing Labgic Technology Co., Ltd.</td><td>BS-200-T</td><td>200 &mu;L</td></tr><tr><td>PIPETTIPJES</td><td>Beijing Labgic Technology Co., Ltd.</td><td>T-1000-B</td><td>1000 &mu;L</td></tr><tr><td>Fenol</td><td>Chengdu Kelong Chemical Products Co., Ltd.</td><td>CAS 108-95-2</td><td></td></tr><tr><td>Roterende verdamper</td><td>&nbsp;BUCHI Laboratory Equipment Trading (Shanghai) Ltd.</td><td>Buchi R-100</td><td></td></tr><tr><td>Standaard Reagens Type Zuiver Water Systeem</td><td>Qingdao Flom Technology Co., Ltd.</td><td>FBZ2001-up-p</td><td></td></tr><tr><td>Thermostaat digitaal weergave waterbad</td><td>Guan'an Scientific Instruments (Zhejiang) Co., Ltd.</td><td>WB100-6F</td><td></td></tr><tr><td>UV-Visible Spectrophotometer</td><td>Shanghai Mapada Instruments Co., Ltd.&nbsp;</td><td>V-1100D</td><td></td></tr><tr><td>Ultrasoonreiniger</td><td>Sheng Zheng ChaoJie Industrial Co.,Ltd.</td><td>CJ-060SD</td><td>Ultrasoonreiniger 360 W 50 Hz</td></tr></tbody></table>

vergelijkende studie van het gehalte aan polysachariden en de antioxiderende werking van hippophae rhamnoides subsp. sinensis en hippophae gyantsensis

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Vergelijkende studie van het gehalte aan polysachariden en de antioxiderende werking van Hippophae rhamnoides subsp. sinensis en Hippophae gyantsensis

This paper reports a well-designed and in-depth comparative study on the polysaccharide yields, contents, and antioxidant activities of two Hippophae ...

comparative-study-on-polysaccharide-contents-antioxidant-activities

Research

JoVE Journal

Chemistry

Scheikunde

Presenteren we een protocol voor het gebruik van ethanol als oplosmiddel te halen en karakteriseren van Taiwanese groene propolis dat antibacteriële activiteit vertoont.

Extractie en analyse van Taiwanese groene Propolis

Fenolzuren zijn belangrijke fytochemicaliën die aanwezig zijn in volle granen. Ze bezitten bioactieve eigenschappen zoals antioxiderende beschermende functies. Dit werk was gericht op het rapporteren over een gegeneraliseerde methode voor de HPLC-identificatie, schatting van het totale fenolgehalte en bepaling van de antioxidantcapaciteit van fenolzuren in granen en peulvruchten.

Een gegeneraliseerde methode voor het bepalen van de vrij oplosbare fenolzuursamenstelling en antioxidantcapaciteit van granen en peulvruchten

Het doel van dit protocol is om fenolische metabolieten in plasma te detecteren met behulp van een semi-gerichte chromatografie-massaspectrometriemethode.

Semi-gerichte ultra-high-performance chromatografie gekoppeld aan massaspectrometrie analyse van fenolische metabolieten in plasma van oudere volwassenen

Het huidige protocol beschrijft de zuiveringsstappen en daaropvolgende studies van vier verschillende schimmel-β-glucanen als potentiële immunomodulerende moleculen die de anti-tumorale eigenschappen van microglia tegen glioblastoomcellen verbeteren.

Isolatie en zuivering van schimmel β-glucaan als immunotherapiestrategie voor glioblastoom

Hier introduceren we een stabiliteitsevaluatiemethode op basis van meerdere lichtverstrooiingstechnologie om de stabiliteit van traditionele Chinese geneeskunde-extracten te evalueren.

Multiple Light Scattering gebruiken om de stabiliteit van Phyllanthus emblica L. Extracten verkregen met verschillende extractiemethoden te onderzoeken

Hier beschrijven we verschillende protocollen die gericht zijn op een geïntegreerde valorisatie van Gracilaria gracilis: het oogsten van wilde soorten, in-house groei en extractie van bioactieve ingrediënten. De antioxiderende, antimicrobiële en cytotoxische effecten van de extracten worden geëvalueerd, samen met de voedings- en stabiliteitsbeoordeling van voedsel verrijkt met hele zeewierbiomassa en pigmenten.

Valorisatie van het rode zeewier Gracilaria gracilis door middel van een bioraffinage-aanpak

Dit protocol beschrijft het gebruik van een op polyol gebaseerde microgolf-ondersteunde extractiemethode voor het extraheren van fenolische verbindingen en natuurlijke antioxidanten, wat een praktische en ecologisch duurzame benadering vertegenwoordigt voor de ontwikkeling van kant-en-klare extracten.

Microgolf-geassisteerde extractie van fenolische verbindingen en antioxidanten voor cosmetische toepassingen met behulp van technologie op basis van polyol

Zoogdieren omhulsel bevat oplosmiddel-extraheerbare lipiden die chemische samenstelling kenmerkend is voor individuele soorten kan bieden. Dit document presenteert een routinemethode voor het scheiden brede lipideklassen Integumentair geïsoleerd uit weefsels met behulp van dunnelaagchromatografie en waarin de triacylglyceride profiel door matrix-geassisteerde laser desorptie / ionisatie time-of-flight massaspectrometrie.

Profilering de triacylglyceride Inhoud in Bat Integumentary Lipids door preparatieve Dunnelaagchromatografie en MALDI-TOF massaspectrometrie

Driedimensionale (3D) microstructured samengestelde liggers zijn gefabriceerd door middel van de gerichte en lokale infiltratie van nanocomposieten in 3D poreuze microfluïdische netwerken. De flexibiliteit van deze produktiemethode maakt het gebruik van verschillende thermohardende materialen en vulstof teneinde verschillende functionele 3D versterkte nanocomposiet macroscopische producten te verbeteren.

Productie van drie-dimensionaal Microgestructureerde Nanocomposieten door Microfluidic Infiltratie

Indoxyl glycosides zijn goed gevestigde en veelgebruikte hulpmiddelen voor enzymscreening en het bewaken van enzymactiviteit. Vooral voor glucose-type structuren bleken eerdere syntheses uitdagend en met een lage opbrengst. Onze nieuwe aanpak gebruikt indoxylzuur esters als waardevolle tussenproducten om een aanzienlijk aantal indoxyl glycosides met een goede opbrengst te verkrijgen.

Synthese van Indoxyl-glycosiden voor detectie van Glycosidase Activiteiten

Ontdek de vergelijking van polysachariden en antioxiderende werking bij Hippophae rhamnoides subsp. sinensis en Hippophae gyantsensis. Deze methode helpt onderzoekers bij kwaliteitsbeoordeling voor farmaceutische toepassingen.

Vergelijkende studie van het gehalte aan polysachariden en de antioxiderende werking van Hippophae rhamnoides subsp. sinensis en Hippophae gyantsensis (Video) | JoVE Journal

Vergelijkende studie van het gehalte aan polysachariden en de antioxiderende werking van Hippophae rhamnoides subsp. sinensis en Hippophae gyantsensis

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