Name: Een efficiënte en snelle HPLC-QQQ-MS-methode voor de kwantificatie van tropaanalkaloïden in medicinale planten (Video) | JoVE Journal
Uploaded: 2026-06-12T13:00:00.000+00:00
Duration: 9 min 10 s
Description: Leer een snelle HPLC-QQQ-MS-methode voor de kwantificatie van tropaanalkaloïden in Anisodus tanguticus. Dit protocol biedt hoge gevoeligheid en reproduceerbaarheid voor farmaceutisch onderzoek.

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<table><tbody><tr><td>Agilent MassHunter software</td><td>Agilent Technologies</td><td></td><td></td></tr><tr><td>Agilent ZORBAX SB-C18 kolom</td><td>Agilent Technologies</td><td>880975-902</td><td>C18 omgekeerde fase kolom, 4,6 &times; 250 mm, 5 &mu;m</td></tr><tr><td>Anisodamine hydrobromide</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>101-31-5</td><td>HPLC>98%</td></tr><tr><td>Formic Acid</td><td>Sigma-Aldrich</td><td>5.33002</td><td></td></tr><tr><td>vriesdroger</td><td>EYELA, Tokyo, Japan</td><td>FDU-2110</td><td></td></tr><tr><td>Hogesnelheid universele molen</td><td>Taisite Instrument Co., Ltd., Tianjin, China</td><td>6010210100</td><td>FW-100, 24.000 rpm, 100 g capaciteit, 80 mesh</td></tr><tr><td>Hyoscyamine</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>55449-49-5</td><td>HPLC>98%</td></tr><tr><td>Methanol</td><td>Thermo Fisher Scientific</td><td>022909.K2</td><td></td></tr><tr><td>Microporeuze membraan 0,22&mu;m</td><td>Millipore</td><td>SLGV033RB</td><td></td></tr><tr><td>Milli-Q Direct 8 watersysteem</td><td>MilliporeSigma, Burlington, MA, USA</td><td>ZRQSVP0JP</td><td></td></tr><tr><td>Scopolamine hydrobromide</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>114-49-8</td><td>HPLC>98%</td></tr></tbody></table>

een efficiënte en snelle hplc-qqq-ms-methode voor de kwantificatie van tropaanalkaloïden in medicinale planten

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

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We presenteren een methode voor snelle massaspectrometrie (MS)/massaspectrometrie (MS)-gebaseerde annotatie en classificatie van tropaanalkaloïden, nuttig voor zowel voorlopige dereplicatie van tropane-bevattende monsters als de ontdekking van nieuwe alkaloïden voor isolatie.

Toepassingen van vloeistofchromatografie tandem massaspectrometrie in onderzoek naar natuurlijke producten: tropaanalkaloïden als casestudy

Dit protocol presenteert een gevalideerde massaspectrometriemethode voor vloeistofchromatografie-ionenmobiliteit en hoge resolutie om de aanwezigheid van moederkorenalkaloïden in voedsel te bepalen in overeenstemming met de onlangs uitgebrachte Verordening (EU) 2023/915 van de Commissie.

Detectie van gereguleerde moederkorenalkaloïden in voedselmatrices door middel van vloeistofchromatografie-ingesloten ionenmobiliteitsspectrometrie-time-of-flight massaspectrometrie

Dit protocol geeft de belangrijkste stappen voor de bioconjugatie van een cysteïne bevattend eiwit een maleïmide, zoals zuivering reagens, reactieomstandigheden, bioconjugaat zuivering en karakterisering bioconjugaat.

Eiwitsynthese Bioconjugaten<em&gt; via</em&gt; Cysteïne-maleïmide Chemistry

We beschrijven de katalytische invoegpolymeervorming van 5-norborneen-2-carbonzuur en 5-vinyl-2-norborneen om functionele polymeren te vormen met een zeer hoge glasovergangstemperatuur.

Een eenvoudige en efficiënte Protocol voor de katalytische Insertion polymerisatie van Functional norbornenen

Een gedetailleerde procedure voor de synthese van een 125 I-gemerkt azide en het radioactief merken van dibenzocyclooctyne (DBCO) -groep geconjugeerd, 13-nm-sized goud nanodeeltjes met behulp kopervrij click reactie wordt beschreven.

Een geoptimaliseerde protocol voor de efficiënte Radioactief merken van Gold Nanodeeltjes met behulp van een<sup&gt; 125</sup&gt;-Gemerkte azide prosthetische groep

Leer een snelle HPLC-QQQ-MS-methode voor de kwantificatie van tropaanalkaloïden in Anisodus tanguticus. Dit protocol biedt hoge gevoeligheid en reproduceerbaarheid voor farmaceutisch onderzoek.

Een efficiënte en snelle HPLC-QQQ-MS-methode voor de kwantificatie van tropaanalkaloïden in medicinale planten

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