Name: ligand binding and allosteric effects
Uploaded: 2023-04-30T18:28:41.000Z
Duration: 49 s
Channel: JoVE

K12 Standards

north-carolina-k-12-science-standard-course-of-study

North Carolina K-12 Science Standard Course of Study

9-12

Biology

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms- Structures and Processes

LS.Bio.1.2

Ligand Binding and Linkage

Większość białek ma wiele lokalizacji, w których ligandy mogą się łączyć, oprócz miejsca odpowiedzialnego za funkcję białka. Wiązanie cząsteczki z którymkolwiek z tych miejsc często powoduje zmiany konformacyjne, zmieniające kształt białka.
Gdy ta zmiana wpływa na wiązanie innego liganda, oba miejsca są opisywane jako sprzężone lub połączone. Powiązanie z miejscem wiązania może być regulowane pozytywnie lub negatywnie.
W wiązaniu dodatnim wiązanie jednego liganda powoduje zmiany konformacyjne, które zwiększają prawdopodobieństwo związania się innego miejsca z odpowiednim ligandem. 
W wiązaniu ujemnym wiązanie jednego liganda powoduje zmiany konformacyjne, które uniemożliwiają innemu miejscu wiązania asocjację z jego ligandem.

<ol>
	<li>Suplatov, D., & Švedas, V. (2015). Badanie miejsc funkcjonalnych i allosterycznych w nadrodzinach białek. Acta naturae, 7(4), 34–45.</li>
	<li>Bellelli, A. i Carey, J. (2017). Białka z wieloma miejscami wiązania. W odwracalnym wiązaniu ligandów (red. A. Bellelli i J. Carey). doi:10.1002/9781119238508.ch4</li>
	<li>Helmstaedt, K., Krappmann, S., & Braus, G. H. (2001). Allosteryczne regulowanie aktywności katalitycznej: transkarbamoylaza asparaginianu Escherichia coli w porównaniu z mutazą choryzmianową drożdży. Przeglądy mikrobiologii i biologii molekularnej : MMBR, 65(3), 404–421. doi:10.1128/MMBR.65.3.404-421.2001</li>
</ol>

wiązanie i wiązanie ligandów

Białka allosteryczne mają więcej niż jedno miejsce wiązania liganda; wiązanie liganda z którymkolwiek z tych miejsc wpływa na wiązanie ligandów z innymi miejscami. Kiedy białko jest allosteryczne, jego miejsca wiązania nazywane są sprzężonymi lub połączonymi. W przypadku enzymów miejsce, które wiąże się z substratem, nazywane jest miejscem aktywnym, a drugie miejsce jest znane jako miejsce regulatorowe. Kiedy ligand wiąże się z miejscem regulatorowym, prowadzi to do zmian konformacyjnych w białku, które mogą wpływać na miejsce aktywne białka. Wiązania miejsc wiązania mogą powodować pozytywną lub negatywną regulację wiązania ligandu z innymi miejscami. W przypadkach, gdy oba ligandy wolą wiązać się z tą samą konformacją białka, wiązanie w jednym miejscu zwiększa powinowactwo drugiego miejsca do odpowiedniego liganda. Nazywa się to pozytywnym powiązaniem. Z drugiej strony, jeśli ligandy wolą wiązać się z różnymi konformacjami, wiązanie jednego liganda utrudni drugiemu ligandowi związanie się z białkiem. Nazywa się to powiązaniem negatywnym.

Wiązanie i wiązanie ligandów

Białka allosteryczne mają więcej niż jedno miejsce wiązania ligandów; Wiązanie liganda z którymkolwiek z tych miejsc wpływa na wiązanie ligandów z innymi ...

Education

JoVE Core

Cell Biology

Biologia komórki

Unit 1

Jednostka 1

Energy and Catalysis

Energia i kataliza

KEY TERMS AND CONCEPTS

Kluczowe terminy i pojęcia

ligand binding and allosteric effects

Ligand binding and linkage describe how one molecule can affect another when it binds to a protein. In this topic, the focus is on how binding at one site can influence activity at a different site on the same protein.

This idea is important for understanding protein function in high school biology. It helps explain why some proteins respond more strongly after a ligand attaches. The transcript centers on the connection between ligand binding and linkage, which is a key concept in molecular interactions.

The term ligand means a molecule that binds to a protein. Linkage describes the way that this binding is connected to changes elsewhere in the protein. Together, these ideas show how protein behavior can be controlled by molecular binding events.

Ligand Binding and Allosteric Effects

the first law of thermodynamics

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Learn how ligand binding can change protein activity through linkage, where binding at one site affects another site on the same protein.

Ligand Binding and Allosteric Effects | JoVE K12

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JoVE Core Cell Biology illustrates complex cellular processes in easy-to-understand yet comprehensive animated video lessons. This collection focuses on the essential components and functions of cells, including organelles and membranes, cellular transport, and cell energetics. It is complemented by scientist-in-action videos that demonstrate the application of cell biology in real-world research experiments.

Virginia  Standards of Learning

HIgh School

BIO.2

BIO.2.c

Florida Department of Education

High School

Life Science

SC.912.L.18

SC.912.L.18.4

SC.912.L.18.11

Texas Essential Knowledge and Skills

BIO.11

BIO.11.B

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Ligand Binding and Allosteric Effects

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