9.3
Rybosomów
Rybosomy tłumaczą informację genetyczną zakodowaną przez informacyjny RNA (mRNA) na białka. Zarówno komórki prokariotyczne, jak i eukariotyczne mają r…
The subunits of eukaryotic ribosomes are protein-synthesizing structures formed inside the nucleolus.
The eukaryotic ribosome is called an 80S ribosome. It is made up of two subunits: a large 60S subunit and a small 40S subunit.
The 60S subunit consists of 28S, 5.8S, and 5S rRNAs and about 49 proteins.
The 40S subunit consists of an 18S rRNA and about 33 proteins.
Both subunits are exported from the nucleus through the nuclear pores. They join on an mRNA to make proteins—either free in the cytosol or attached to the outer nuclear envelope or the rough endoplasmic reticulum.
The mRNA binding site is located in the small subunit. The three tRNA binding sites, E, P, and A, are formed at the interface of the two subunits.
During translation, the initiator tRNA binds directly to the P, or peptidyl, site. During the elongation stage, new tRNAs enter the A or aminoacyl site, and empty tRNAs leave from the E or exit site.
The rRNA in the large subunit also catalyzes peptide bond formation between amino acids, allowing the growing polypeptide chain to form.
View the full transcript and gain access to JoVE Core videos
Q1: What is the basic structure and function of a ribosome?
Ribosomes are cellular machines composed of ribosomal RNA and proteins that synthesize proteins by translating messenger RNA sequences into amino acid chains. They consist of two subunits that work together to read the genetic code and catalyze peptide bond formation between amino acids during protein synthesis.
Q2: How do ribosomes recognize and use the genetic code during translation?
Ribosomes read messenger RNA codons through transfer RNA molecules that carry specific amino acids. Each tRNA has an anticodon that pairs with the corresponding mRNA codon, ensuring the correct amino acid is added at each step. This codon-anticodon matching is fundamental to accurate protein synthesis.
Q3: What role does the peptidyl transferase center play in protein synthesis?
The peptidyl transferase center is the catalytic site within the ribosome where peptide bonds form between adjacent amino acids. This enzymatic region catalyzes the transfer of the growing polypeptide chain from one tRNA to the next, enabling chain elongation. Understanding improving translational accuracy helps explain how this process maintains fidelity.
Q4: What happens during the initiation phase of translation?
Translation initiation involves the ribosome assembling at the start codon of messenger RNA with the help of initiation factors. The first tRNA carrying methionine binds to establish the reading frame. This phase establishes the foundation for accurate protein synthesis by positioning the ribosome correctly on the mRNA template.
Q5: How does translation end and what triggers ribosome release?
Translation terminates when the ribosome encounters a stop codon on the messenger RNA. Release factors recognize these stop signals and catalyze hydrolysis of the polypeptide chain from the final tRNA. The ribosome then dissociates from the mRNA, completing protein synthesis and allowing the newly formed protein to undergo further processing.
Q6: What quality control mechanisms ensure accurate protein synthesis by ribosomes?
Ribosomes employ multiple accuracy checkpoints, including proofreading during tRNA selection and monitoring of codon-anticodon pairing. The ribosome can reject incorrect tRNAs before peptide bond formation occurs. These mechanisms minimize errors in translation, maintaining the fidelity of genetic information transfer from DNA to functional proteins.
Q7: How do molecular chaperones assist newly synthesized proteins after ribosomal synthesis?
After ribosomes release newly synthesized polypeptide chains, molecular chaperones and protein folding systems help guide proper three-dimensional structure formation. These proteins prevent aggregation and ensure the nascent chain folds into its functional conformation. This post-translational assistance is critical for producing active, properly folded proteins.